Smith A R, van Alphen B, Faithfull N S, Fennema M
Plast Reconstr Surg. 1985 Feb;75(2):227-37. doi: 10.1097/00006534-198502000-00015.
Five patients are presented in whom a traumatically amputated extremity or digit was perfused with oxygenated Fluosol DA-20%. Perfusion lasted between 16 and 46 hours. Microcirculatory oxygenation was monitored using pO2 measurements. Moreover, K¿lactate, glucose, and ATP levels were measured and electron microscopic sections of the vessels were investigated at the end of the experiment. All above-mentioned parameters suggest that the tissue was sufficiently provided with oxygen and viable after many hours of perfusion. Conclusive evidence of the viability of a perfused digit was given by its successful replantation after 17 hours of Fluosol perfusion (at approximately 15 degrees C).
本文介绍了5例患者，他们因创伤导致肢体或手指离断后接受了20%的含氧氟碳化合物（Fluosol DA）灌注。灌注持续时间为16至46小时。使用pO2测量来监测微循环氧合。此外，在实验结束时测量了乳酸钾、葡萄糖和ATP水平，并对血管进行了电子显微镜切片研究。所有上述参数表明，经过数小时的灌注后，组织获得了充足的氧气供应且保持存活。在氟碳化合物灌注17小时后（约15摄氏度），成功进行了断指再植，这为灌注手指的存活提供了确凿证据。
