Ren Ke, Wang Yi, Sun Tao, Yue Wen, Zhang Hongyu
School of Engineering and Technology, China University of Geosciences (Beijing), Beijing 100083, China.
State Key Laboratory of Tribology, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, China.
Mater Sci Eng C Mater Biol Appl. 2017 Sep 1;78:324-332. doi: 10.1016/j.msec.2017.04.084. Epub 2017 Apr 17.
Guided bone regeneration (GBR) membranes have been proved of great benefit for bone tissue engineering due to the improvement of cell attachment and proliferation. To develop GBR membranes with better biocompatibility and more proper degradation ability, here we fabricated polycaprolactone (PCL, polymer)/gelatin (protein) hybrid nanofibrous GBR membranes via electrospinning, followed by crosslinking with genipin. Acetic acid (HAc) was utilized to resolve the phase separation of PCL and gelatin, therefore homogeneous PCL/gelatin hybrid nanofibers with different ratios were successfully prepared. FTIR, XPS, TGA, DSC results proved that the proportion of PCL and gelatin in the as-spun nanofiber membranes could be simply adjusted by changing the weight ratio of PCL and gelatin in the spinning solution. SEM and AFM images demonstrated that all the nanofibers possessed uniform and smooth structures both in two dimension (2D) and three dimension (3D). The mechanical tests showed that these nanofibers exhibited appropriate tensile and strength properties, which were suitable for bone tissue engineering. CCK-8 and SEM images revealed that all the membranes were biocompatible to MC3T3-e1 cells. In addition, the in vitro osteogenesis characterizations, alizarin red in normal medium and osteogenesis medium, indicated that the nanofibers could promote bone formation. Therefore, all these results could suggest that our design of electrospun polymer/protein nanofiber membranes was effective for guided bone regeneration.
引导骨再生（GBR）膜已被证明对骨组织工程有很大益处，因为它能改善细胞附着和增殖。为了开发具有更好生物相容性和更合适降解能力的GBR膜，我们通过静电纺丝制备了聚己内酯（PCL，聚合物）/明胶（蛋白质）混合纳米纤维GBR膜，然后用京尼平进行交联。利用乙酸（HAc）解决PCL和明胶的相分离问题，从而成功制备了不同比例的均匀PCL/明胶混合纳米纤维。傅里叶变换红外光谱（FTIR）、X射线光电子能谱（XPS）、热重分析（TGA）、差示扫描量热法（DSC）结果证明，通过改变纺丝溶液中PCL和明胶的重量比，可以简单地调整初纺纳米纤维膜中PCL和明胶的比例。扫描电子显微镜（SEM）和原子力显微镜（AFM）图像表明，所有纳米纤维在二维（2D）和三维（3D）中均具有均匀且光滑的结构。力学测试表明，这些纳米纤维表现出合适的拉伸和强度性能，适用于骨组织工程。细胞计数试剂盒-8（CCK-8）和SEM图像显示，所有膜对MC3T3-e1细胞均具有生物相容性。此外，体外成骨特性，即在正常培养基和成骨培养基中的茜素红染色，表明纳米纤维可以促进骨形成。因此，所有这些结果表明，我们设计的静电纺丝聚合物/蛋白质纳米纤维膜对引导骨再生是有效的。
