Department of General and Digestive Surgery, "Virgen del Rocío" University Hospital/IBiS/CSIC/University of Seville, 41013 Seville, Spain.
Oncology Surgery, Cell Therapy, and Organ Transplantation Group. Institute of Biomedicine of Seville (IBiS), "Virgen del Rocío" University Hospital, IBiS, CSIC/University of Seville, 41013 Sevilla, Spain.
ACS Appl Bio Mater. 2024 Oct 21;7(10):6430-6440. doi: 10.1021/acsabm.4c00559. Epub 2024 Oct 4.
The current treatments to restore skeletal muscle defects present several injuries. The creation of scaffolds and implant that allow the regeneration of this tissue is a solution that is reaching the researchers' interest. To achieve this, electrospinning is a useful technique to manufacture scaffolds with nanofibers with different orientation. In this work, polycaprolactone and gelatin solutions were tested to fabricate electrospun scaffolds with two degrees of alignment between their fibers: random and aligned. These scaffolds can be seeded with myoblast C2C12 and then stimulated with a mechanical bioreactor that mimics the physiological conditions of the tissue. Cell viability as well as cytoskeletal morphology and functionality was measured. Myotubes in aligned scaffolds (9.84 ± 1.15 μm) were thinner than in random scaffolds (11.55 ± 3.39 μm; = 0.001). Mechanical stimulation increased the width of myotubes (12.92 ± 3.29 μm; < 0.001), nuclear fusion (95.73 ± 1.05%; = 0.004), and actin density (80.13 ± 13.52%; = 0.017) in aligned scaffolds regarding the control. Moreover, both scaffolds showed high myotube contractility, which was increased in mechanically stimulated aligned scaffolds. These scaffolds were also electrostimulated at different frequencies and they showed promising results. In general, mechanically stimulated aligned scaffolds allow the regeneration of skeletal muscle, increasing viability, fiber thickness, alignment, nuclear fusion, nuclear differentiation, and functionality.
目前，用于修复骨骼肌缺陷的治疗方法存在多种损伤。制造支架和植入物以促进该组织再生是一种引起研究人员兴趣的解决方案。为此，静电纺丝是一种制造具有不同取向纳米纤维支架的有用技术。在这项工作中，测试了聚己内酯和明胶溶液，以制造纤维之间具有两种对准度的静电纺丝支架：随机和对准。可以将这些支架接种 C2C12 成肌细胞，然后用模拟组织生理条件的机械生物反应器进行刺激。测量了细胞活力以及细胞骨架形态和功能。与随机支架（11.55 ± 3.39 μm）相比，定向支架（9.84 ± 1.15 μm； = 0.001）中的肌管更薄。机械刺激增加了肌管的宽度（12.92 ± 3.29 μm； < 0.001）、核融合（95.73 ± 1.05%； = 0.004）和肌动蛋白密度（80.13 ± 13.52%； = 0.017），与对照相比，定向支架中的核融合（95.73 ± 1.05%； = 0.004）和肌动蛋白密度（80.13 ± 13.52%； = 0.017）。此外，两种支架均显示出较高的肌管收缩性，机械刺激定向支架中的肌管收缩性增加。这些支架还在不同频率下进行了电刺激，结果令人鼓舞。总的来说，机械刺激的定向支架可促进骨骼肌再生，提高细胞活力、纤维厚度、定向性、核融合、核分化和功能。
