Ricci R, Leite N C S, da-Silva N S, Pacheco-Soares C, Canevari R A, Marciano F R, Webster T J, Lobo A O
Laboratory of Biomedical Nanotechnology, Research and Development Institute, University of Vale do Paraiba, Av. Shishima Hifumi, 2911, CEP: 12244-000 São José dos Campos, SP, Brazil.
Laboratory of Cell and Tissue Biology, Research and Development Institute, University of Vale do Paraiba, Av. Shishima Hifumi, 2911, CEP: 12244-000 São José dos Campos, SP, Brazil.
Mater Sci Eng C Mater Biol Appl. 2017 Sep 1;78:341-348. doi: 10.1016/j.msec.2017.03.278. Epub 2017 Apr 13.
Graphene oxide nanoribbons (O-GNR) surges as an interesting nanomaterial for biomedical applications due to feasibility to incorporate functional groups and possible bactericidal properties. Herein, high concentrations of O-GNR were biologically evaluated using human osteoblast cells and gram positive and negative bacteria. Briefly, our goal were to evaluate: (1) synthetic pathway, (2) characterization and (3) effects of O-GNR composition and structural factors as a new approach for biomedical applications. For this, O-GNR were produced combining chemical vapor deposition and oxygen plasma treatment of multiwalled carbon nanotubes. Then, we analyzed the bioactivity, cell viability, osteogenic differentiation, matrix mineralization, mRNA levels of the five genes related direct to bone repair and bactericidal effect of high concentrations of O-GNR (10μgmL, 100μgmL, 200μgmL and 300μgmL). Impressively, O-GNR showed no cytotoxic effects up to a concentration of 100μgmL and no gene expression alteration when used in its dose. We also observed that S. aureus and E. coli bacteria are susceptible to damage when incubated with 100μgmL of O-GNR, showing approximately 50% of bacterial death. We consider that O-GNR displays attractive properties when used at a suitable dose, displaying bactericidal effect and apparently lacking to cause damages in the bone repair process.
氧化石墨烯纳米带（O-GNR）作为一种有趣的纳米材料在生物医学应用中崭露头角，这是因为它能够引入官能团且可能具有杀菌特性。在此，我们使用人成骨细胞以及革兰氏阳性和阴性细菌对高浓度的O-GNR进行了生物学评估。简而言之，我们的目标是评估：（1）合成途径，（2）表征，以及（3）O-GNR的组成和结构因素作为生物医学应用新方法的效果。为此，通过化学气相沉积和对多壁碳纳米管进行氧等离子体处理来制备O-GNR。然后，我们分析了高浓度O-GNR（10μg/mL、100μg/mL、200μg/mL和300μg/mL）的生物活性、细胞活力、成骨分化、基质矿化、与骨修复直接相关的五个基因的mRNA水平以及杀菌效果。令人印象深刻的是，O-GNR在浓度高达100μg/mL时未显示出细胞毒性作用，并且在其使用剂量下未引起基因表达改变。我们还观察到，当与100μg/mL的O-GNR孵育时，金黄色葡萄球菌和大肠杆菌易受损伤，显示出约50%的细菌死亡。我们认为，O-GNR在以合适剂量使用时具有吸引人的特性，具有杀菌作用且显然不会在骨修复过程中造成损害。
