Kannan Ayyadurai, Sevvel Ranganathan
Post Graduate and Research Department of Chemistry, Vivekananda College, Tiruvedakam West, Madurai 625 234, Tamil Nadu, India.
Post Graduate and Research Department of Chemistry, Vivekananda College, Tiruvedakam West, Madurai 625 234, Tamil Nadu, India.
Mater Sci Eng C Mater Biol Appl. 2017 Sep 1;78:513-519. doi: 10.1016/j.msec.2017.04.105. Epub 2017 Apr 19.
This paper demonstrates the fabrication of novel gold nanoparticles incorporated poly (4-amino-6-hydroxy-2-mercaptopyrimidine) (Nano-Au/Poly-AHMP) film modified glassy carbon electrode and it is employed for highly sensitive detection of l-cysteine (CYS). The modified electrode was characterized by scanning electron microscope (SEM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). SEM images of modified electrode revealed the homogeneous distribution of gold nanoparticles on poly (4-amino-6-hydroxy-2-mercaptopyrimidine) thin film modified glassy carbon electrode. The modified electrode was successfully utilized for highly selective and sensitive determination of l-cysteine at physiological pH7.0. The present electrochemical sensor successfully resolved the voltammetric signals of ascorbic acid (AA) and l-cysteine with peak separation of 0.510V. To the best of our knowledge, this is the first report of larger peak separation between AA and CYS. Wide linear concentration ranges (2μM-500μM), low detection limit (0.020μM), an excellent reproducibility and stability are achieved for cysteine sensing with this Nano-Au/Poly-AHMP/GCE.
本文展示了新型金纳米粒子掺杂聚（4-氨基-6-羟基-2-巯基嘧啶）（纳米金/聚-AHMP）膜修饰玻碳电极的制备，并将其用于高灵敏度检测 l-半胱氨酸（CYS）。通过扫描电子显微镜（SEM）、循环伏安法（CV）和电化学阻抗谱（EIS）对修饰电极进行了表征。修饰电极的 SEM 图像显示金纳米粒子均匀分布在聚（4-氨基-6-羟基-2-巯基嘧啶）薄膜修饰的玻碳电极上。该修饰电极成功用于在生理 pH7.0 下高选择性和灵敏地测定 l-半胱氨酸。本电化学传感器成功分辨了抗坏血酸（AA）和 l-半胱氨酸的伏安信号，峰分离度为 0.510V。据我们所知，这是关于 AA 和 CYS 之间有较大峰分离度的首次报道。使用这种纳米金/聚-AHMP/玻碳电极进行半胱氨酸传感，实现了宽线性浓度范围（2μM - 500μM）、低检测限（0.020μM）、出色的重现性和稳定性。
