Research Center for Analytical Sciences, College of Chemistry, Tianjin Key Laboratory of Biosensing and Molecular Recognition, State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin 300071, China.
Research Center for Analytical Sciences, College of Chemistry, Tianjin Key Laboratory of Biosensing and Molecular Recognition, State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin 300071, China; Collaborative Innovation Center of Chemical Science and Engineering, Nankai University, Tianjin 300071, China.
Talanta. 2018 Apr 1;180:54-60. doi: 10.1016/j.talanta.2017.12.037. Epub 2017 Dec 14.
An efficient glycoproteins enrichment platform is one of vital preprocessing steps in biomarker research and in particular glycoproteomics. In this work, a well-defined boronic acid functionalized magnetic graphene oxide nanocomposite (FeO-GO@PAAPBA) was synthesized for the selective enrichment of glycoproteins from complex biological samples via a novel strategy based on the "thiol-ene" click chemistry and surface initiated atom transfer radical polymerization (SI-ATRP). The initiator of ATRP was anchored to the surface of substrate through "thiol-ene" click reaction. The product FeO-GO@PAAPBA was successfully synthesized in following SI-ATRP. The FeO-GO@PAAPBA nanocomposite was characterized by transmission electron microscopy (TEM), Fourier transform-infrared spectroscopy (FTIR), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), vibrating sample magnetometry (VSM) and thermogravimetric analysis. The adsorption capacity of FeO-GO@PAAPBA towards ovalbumin (OVA) and transferrin (Trf) is 471mgg and 450mgg, respectively. The nanocomposite also featured good selectivity to glycoproteins in the mixture of glycoproteins and non-glycoproteins at alkaline (pH 9.0) and physiological conditions (pH 7.4). Furthermore, it can be applied to extract glycoproteins directly from egg white samples. These results have indicated that FeO-GO@PAAPBA was a potential affinity material in glycoprotein analysis.
一种高效的糖蛋白富集平台是生物标志物研究，特别是糖蛋白质组学的关键预处理步骤之一。在这项工作中，通过基于“硫醇-烯”点击化学和表面引发原子转移自由基聚合（SI-ATRP）的新策略，合成了一种精确定义的硼酸功能化磁性氧化石墨烯纳米复合材料（FeO-GO@PAAPBA），用于从复杂生物样品中选择性富集糖蛋白。ATRP 的引发剂通过“硫醇-烯”点击反应锚定在基底表面上。随后通过 SI-ATRP 成功合成了产物 FeO-GO@PAAPBA。通过透射电子显微镜（TEM）、傅里叶变换红外光谱（FTIR）、X 射线衍射（XRD）、X 射线光电子能谱（XPS）、振动样品磁强计（VSM）和热重分析对 FeO-GO@PAAPBA 纳米复合材料进行了表征。FeO-GO@PAAPBA 对卵清蛋白（OVA）和转铁蛋白（Trf）的吸附容量分别为 471mgg 和 450mgg。该纳米复合材料在碱性（pH9.0）和生理条件（pH7.4）下的糖蛋白和非糖蛋白混合物中也具有良好的选择性。此外，它还可以直接从蛋清样品中提取糖蛋白。这些结果表明，FeO-GO@PAAPBA 是糖蛋白分析中一种有潜力的亲和材料。
