Polymer Biomaterial Design and Synthesis Laboratory, Department of Chemistry, Yogi Vemana University, Kadapa, Andhra Pradesh, 516003, India.
Advanced Nanomaterials Lab, Department of Polymer Science and Engineering, Pusan National University, Busan 46241, South Korea.
Int J Biol Macromol. 2017 Oct;103:1162-1172. doi: 10.1016/j.ijbiomac.2017.05.160. Epub 2017 May 30.
Potential dual responsive hydrogel networks (PPAD) are fabricated from pectin, poly((2-dimethylamino)ethyl methacrylate)) and phosphate crosslinker bis[2-methacryloyloxy] ethyl phosphate (BMEP) by a simple free radical polymerization. These hydrogel networks are successfully utilized for encapsulation of an anti-cancer drug, 5-fluorouracil (5-FU) and also employed as versatile platforms for production of silver nanoparticles. Fabricated hydrogel networks and silver nanocomposites were characterized by FTIR, SEM, EDX, TEM, DLS, DSC, TGA and XRD. Different polymer network parameters such as M¯, χ, ξ and υ and diffusion constant (D) were evaluated to assess the drug release profile. The 5FU loaded PPAD hydrogels were used to perform in vitro release studies in both gastric and intestinal conditions of GIT (pH 1.2 & pH 7.4) at two different temperatures (25 and 37°C). On the other hand various kinetic models (zero, first, Higuchi & Koresmeyer-Peppas) have also been employed to fit drug release profile. In addition, the antibacterial activity of PPAD silver nanocomposites were tested against four bacterial species Escherichia coli (-ve), Klebsiella pneumoniae (-ve), Bacillus cereus (+ve) and Staphylococcus aereus (+ve) using zone of inhibition test.
由果胶、聚（2-二甲氨基乙基甲基丙烯酸酯）和磷酸盐交联剂双[2-（甲基丙烯酰氧基）乙基]磷酸酯（BMEP）通过简单的自由基聚合制备潜在的双重响应水凝胶网络（PPAD）。这些水凝胶网络成功地用于封装抗癌药物 5-氟尿嘧啶（5-FU），并且还用作生产银纳米粒子的多功能平台。通过 FTIR、SEM、EDX、TEM、DLS、DSC、TGA 和 XRD 对制备的水凝胶网络和银纳米复合材料进行了表征。评估了不同的聚合物网络参数，如 M¯、χ、ξ 和 υ 以及扩散常数（D），以评估药物释放曲线。负载 5FU 的 PPAD 水凝胶用于在 GIT（pH 1.2 和 pH 7.4）的两种不同温度（25 和 37°C）下进行胃和肠道条件下的体外释放研究。另一方面，还采用了各种动力学模型（零级、一级、Higuchi 和 Koresmeyer-Peppas）来拟合药物释放曲线。此外，还使用抑菌圈试验测试了 PPAD 银纳米复合材料对四种细菌的抗菌活性，包括大肠杆菌（-ve）、肺炎克雷伯菌（-ve）、蜡状芽孢杆菌（+ve）和金黄色葡萄球菌（+ve）。
