Division of Cardiology, Department of Internal Medicine, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA; Eli and Edythe Broad Stem Cell Research Center, University of California, Los Angeles, CA 90095, USA.
Department of Medicine, Division of Cardiovascular Medicine, University of Wisconsin-Madison, Madison, WI 53706, USA.
Trends Mol Med. 2017 Jul;23(7):651-668. doi: 10.1016/j.molmed.2017.05.001. Epub 2017 May 30.
Human pluripotent stem cells (hPSCs) offer a practical source for the de novo generation of cardiac tissues and a unique opportunity to investigate cardiovascular lineage commitment. Numerous strategies have focused on the in vitro production of cardiomyocytes, smooth muscle, and endothelium from hPSCs. However, these differentiation protocols often yield undesired cell types. Thus, establishing a set of stage-specific markers for pure cardiac subpopulations will assist in defining the hierarchy of cardiac differentiation, aid in the development of cellular therapy, and facilitate drug screening and disease modeling. The recent characterization of many such markers is enabling the isolation of major cardiac lineages and subpopulations from differentiating hPSCs. We provide here a comprehensive review detailing the suite of biomarkers used to differentiate cardiac lineages from mixed hPSC-derived populations.
人类多能干细胞 (hPSCs) 为心脏组织的从头生成提供了一个实用的来源，也为研究心血管谱系决定提供了独特的机会。许多策略都集中在从 hPSCs 体外生成心肌细胞、平滑肌和内皮细胞上。然而，这些分化方案往往会产生不理想的细胞类型。因此，建立一套用于纯心脏亚群的阶段特异性标记物将有助于确定心脏分化的层次结构，有助于细胞治疗的发展，并促进药物筛选和疾病建模。最近对许多此类标记物的特征描述，使得可以从分化的 hPSCs 中分离出主要的心脏谱系和亚群。我们在此提供了一个全面的综述，详细介绍了用于从混合 hPSC 衍生群体中分化心脏谱系的生物标志物。
