单侧和双侧Mg2+对线粒体H+-ATP酶重构作用的比较

Comparison of the effects of one-side- and two-side Mg2+ on the reconstitution of mitochondrial H+-ATPase.

作者信息

Yang F Y, Zhang X F

出版信息

FEBS Lett. 1985 Mar 11;182(1):31-3. doi: 10.1016/0014-5793(85)81147-9.

Abstract

Three types of proteoliposome containing mitochondrial H+-ATPase have been prepared: Mg2+-'free', one-side and two-side Mg2+-containing proteoliposomes. The ATPase activity as well as its sensitivity to oligomycin or N,N'-dicyclohexylcarbodiimide of the three proteoliposome preparations has been compared. They decreased in the order: L X (H+-ATPase)+ Mg2+ greater than L X (H+-ATPase)-+ Mg2+ greater than L X (H+-ATPase)-Mg2+. The fluidity of the proteoliposomes has also been compared by fluorescence polarization probes diphenylhexatriene (DPH) or 7-(9-anthroyloxy)stearic acid (7-AS). The degree of polarization for DPH in these proteoliposomes decreased in the order: L X (H+-ATPase) +Mg2+ greater than L X (H+-ATPase)-+ Mg2+ greater than L X (H+-ATPase)-Mg2+, while that for 7-AS: L X (H+-ATPase) +Mg2+ approximately equal to L X (H+-ATPase)-+ Mg2+ greater than L X (H+-ATPase) -Mg2+.

摘要

已制备出三种含有线粒体H⁺-ATP酶的蛋白脂质体：不含Mg²⁺的、单侧含Mg²⁺的和双侧含Mg²⁺的蛋白脂质体。对这三种蛋白脂质体制剂的ATP酶活性及其对寡霉素或N,N'-二环己基碳二亚胺的敏感性进行了比较。它们的活性按以下顺序降低：L X (H⁺-ATP酶)+Mg²⁺＞L X (H⁺-ATP酶)-+Mg²⁺＞L X (H⁺-ATP酶)-Mg²⁺。还通过荧光偏振探针二苯基己三烯（DPH）或7-(9-蒽氧基)硬脂酸（7-AS）对蛋白脂质体的流动性进行了比较。这些蛋白脂质体中DPH的偏振度按以下顺序降低：L X (H⁺-ATP酶)+Mg²⁺＞L X (H⁺-ATP酶)-+Mg²⁺＞L X (H⁺-ATP酶)-Mg²⁺，而7-AS的偏振度顺序为：L X (H⁺-ATP酶)+Mg²⁺≈L X (H⁺-ATP酶)-+Mg²⁺＞L X (H⁺-ATP酶)-Mg²⁺。