Gorskaya Yu F, Nesterenko V G
Laboratory of Immunity Regulation and Immune Tolerance, N. F. Gamaleya Federal Center of Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Moscow, Russia.
Bull Exp Biol Med. 2017 May;163(1):137-141. doi: 10.1007/s10517-017-3754-6. Epub 2017 Jun 3.
At the early stages of development (3 months), transplants from bone marrow donors subjected to single in vivo stimulation (curettage, administration of BMP-2 or antigenic complex of S. typhimurium) 1 day before transplantation were characterized by significantly elevated content of nucleated cells (by 1.4, 1.9 and 2.9 times, respectively), efficiency of cloning of multipotent stromal cells (by 3.8, 3.8 and 7.2 times), and total number of multipotent stromal cells (by 5, 7 and 21 times) and osteogenic multipotent stromal cells (by 5, 9 and 15 times) in comparison with the control (intact donors); more rapid increase in the weight of bone capsules was also noted. At later terms, the difference by these parameters between the control and experimental groups became less pronounced, but even in 4.5 months, the total number of multipotent stromal cells in the transplant in experimental groups exceeded the control values by 1.4-1.7 times and osteogenic multipotent stromal cells by 2 times. In donors exposed to the specified stimulations, the content of multipotent stromal cells in the femoral bone marrow in 1 day increased by 2.1 times (curettage), 2.6 times (administration of S. typhimurium antigens), and 3.3 times (LPS); administration of BMP-2 reduced this value by 50%. The content of osteogenic bone marrow multipotent stromal cells at this term increased by 1.7 times (BMP-2) and 5.5 times (curettage), after administration of S. typhimurium antigens, this parameter corresponded to the control. The concentration of osteogenic multipotent stromal cells in the bone marrow of intact donors was 22%; the maximum values were observed after curettage (57%) and BMP-2 administration (74%) and minimum after treatment with S. typhimurium antigens (8%). However, this parameter in all groups of transplants little differed and leveled as soon as by 3-4 months, which can be due to regulatory influences of the recipient body. The initial advantage in the content of bone marrow multipotent stromal cells in donors exposed to osteogenic stimuli and administration of antigens ensured considerably more rapid growth of the transplants in comparison with the control. These results can be useful for the development of optimal protocols of tissue grafting.
在发育早期阶段（3个月），对骨髓供体在移植前1天进行单次体内刺激（刮除术、给予BMP - 2或鼠伤寒沙门氏菌抗原复合物）后进行的移植，其特征在于有核细胞含量显著升高（分别升高1.4倍、1.9倍和2.9倍），多能间充质细胞克隆效率（分别升高3.8倍、3.8倍和7.2倍），以及多能间充质细胞总数（分别升高5倍、7倍和21倍）和成骨多能间充质细胞数量（分别升高5倍、9倍和15倍），与对照组（未处理的供体）相比；还观察到骨膜重量增加更快。在后期，对照组和实验组在这些参数上的差异变得不那么明显，但即使在4.5个月时，实验组移植中的多能间充质细胞总数仍比对照组值高出1.4 - 1.7倍，成骨多能间充质细胞高出2倍。在接受特定刺激的供体中，股骨骨髓中多能间充质细胞含量在1天内分别增加2.1倍（刮除术）、2.6倍（给予鼠伤寒沙门氏菌抗原）和3.3倍（LPS）；给予BMP - 2使该值降低50%。此时成骨骨髓多能间充质细胞含量分别增加1.7倍（BMP - 2）和5.5倍（刮除术），给予鼠伤寒沙门氏菌抗原后，该参数与对照组相当。未处理供体骨髓中成骨多能间充质细胞的浓度为22%；刮除术（57%）和给予BMP - 2（74%）后观察到最大值，而用鼠伤寒沙门氏菌抗原处理后最小值为8%。然而，所有移植组中的该参数差异不大，并且在3 - 4个月时就趋于平稳，这可能是由于受体机体的调节影响。与对照组相比，接受成骨刺激和抗原给药的供体骨髓多能间充质细胞含量的初始优势确保了移植组织生长明显更快。这些结果对于制定最佳组织移植方案可能有用。
