Department of Pharmacology, Wuhan University School of Basic Medical Sciences, Wuhan, China.
Department of Pharmacology, Wuhan University School of Basic Medical Sciences, Wuhan, China; Hubei Provincial Key Laboratory of Developmentally Originated Diseases, Wuhan, China.
Arch Med Res. 2017 Jan;48(1):35-45. doi: 10.1016/j.arcmed.2017.01.002.
The present study was designed to investigate the effects of prenatal food restriction (PFR) with postweaning high-fat diet (HFD) on glucose metabolic function in adult offspring.
Pregnant Wistar rats were given PFR treatment from gestational day 11 to spontaneous delivery. All pups were fed by HFD after weaning. Oral glucose tolerance test (OGTT) was conducted at postnatal week (PW) 20. Rats were decapitated in PW24 to collect liver and pancreas, and expression of hepatic insulin signaling genes were then quantified.
Body weight from PW4 to PW24 in PFR males was lower than those in control males, whereas there was no distinct difference between females. However, body weight gain rates were higher from PW16 to PW24 in PFR males and females. Fasting serum glucose presented no changes, whereas fasting serum insulin decreased in PW20 in PFR pups. Moreover, glucose intolerance only appeared in PFR males, whereas no changes were shown in PFR females in relative values. Serum insulin increased in both PFR groups after OGTT. Remarkable pathological changes were also found in islets from PFR rats. There was an increase in the hepatic mRNA expression of IR in PFR females and of Glut2 in PFR males.
PFR with postweaning HFD induced a catch-up growth in body weight, especially in PFR females. Serum insulin decreased in both PFR groups in fasting status. Insulin resistance after OGTT only existed in PFR males, whereas PFR females showed no obvious changes in glucose metabolism.
本研究旨在探讨产前限食（PFR）联合断乳后高脂肪饮食（HFD）对成年子代葡萄糖代谢功能的影响。
从妊娠第 11 天至自然分娩，对 Wistar 孕鼠进行 PFR 处理。所有幼鼠在断乳后均给予 HFD 喂养。在出生后第 20 周进行口服葡萄糖耐量试验（OGTT）。在出生后第 24 周，大鼠断头取血，采集肝脏和胰腺，然后定量检测肝胰岛素信号基因的表达。
PFR 雄性大鼠从第 4 周到第 24 周的体重均低于对照组雄性大鼠，而雌性大鼠之间则无明显差异。然而，PFR 雄性和雌性大鼠从第 16 周到第 24 周的体重增长率均较高。空腹血清葡萄糖无变化，而 PFR 幼鼠在第 20 周时空腹血清胰岛素降低。此外，葡萄糖耐量仅在 PFR 雄性大鼠中出现异常，而 PFR 雌性大鼠的相对值则无变化。OGTT 后，两组 PFR 大鼠的血清胰岛素均增加。PFR 大鼠的胰岛也出现了明显的病理变化。PFR 雌性大鼠的肝 IR mRNA 表达增加，PFR 雄性大鼠的 Glut2 表达增加。
PFR 联合断乳后 HFD 可引起体重的追赶性生长，尤其是 PFR 雌性大鼠。两组 PFR 大鼠在空腹状态下血清胰岛素降低。OGTT 后仅 PFR 雄性大鼠出现胰岛素抵抗，而 PFR 雌性大鼠的葡萄糖代谢无明显变化。
