PI3K/AKT/mTOR信号通路在体外DBP诱导睾丸支持细胞凋亡中的作用
Role of PI3K/AKT/mTOR signaling pathway in DBP-induced apoptosis of testicular sertoli cells in vitro.
作者信息
Wang Hongyan, Wang Jun, Zhang Jing, Jin Shuguang, Li Huan
机构信息
Department of Environmental Hygiene, School of Public Health, Beihua University, Jilin 132013, China.
Department of Radiotherapy, The Second People's Hospital of Jilin City, Jilin 132002, China, China.
出版信息
Environ Toxicol Pharmacol. 2017 Jul;53:145-150. doi: 10.1016/j.etap.2017.05.013. Epub 2017 May 31.
Dibutyl phthalate (DBP) has significant male reproductive toxicity, and the Sertoli cells are the target cells of DBP. This study was to investigate the injury effect induced by DBP on rat testicular Sertoli cells in vitro. MTT results showed that DBP can significantly reduce the survival rate of Sertoli cells; Hoechst staining results showed that the Sertoli cells treated with DBP emerged with typical morphological characteristics of apoptosis, nuclear condensation and chromatin condensation; flow cytometry results showed that DBP significantly increased the apoptotic rate of Sertoli cells, and dose-dependent; Western blotting showed that the expression of PTEN protein in Sertoli cells was significantly higher than that in the control group after treated with different concentrations of DBP for 24h, while the expression of p-PI3K1, p-AKT, p70S6K and 4E-BP1 protein in the PI3K/AKT/mTOR signal pathway were significantly decreased. It is speculated that PTEN/PI3K/AKT/mTOR signaling pathway plays an important role in DBP-induced apoptosis of testicular Sertoli cells in rats.
邻苯二甲酸二丁酯（DBP）具有显著的雄性生殖毒性，而支持细胞是DBP的靶细胞。本研究旨在探讨DBP体外诱导大鼠睾丸支持细胞损伤的作用。MTT结果显示，DBP可显著降低支持细胞的存活率；Hoechst染色结果显示，经DBP处理的支持细胞出现典型的凋亡形态特征，细胞核固缩、染色质凝聚；流式细胞术结果显示，DBP显著提高支持细胞的凋亡率，且呈剂量依赖性；蛋白质免疫印迹法显示，不同浓度DBP处理24小时后，支持细胞中PTEN蛋白表达显著高于对照组，而PI3K/AKT/mTOR信号通路中p-PI3K1、p-AKT、p70S6K和4E-BP1蛋白表达显著降低。推测PTEN/PI3K/AKT/mTOR信号通路在DBP诱导的大鼠睾丸支持细胞凋亡中起重要作用。
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