Diba Mani, Pape Bram, Klymov Alexey, Zhang Yang, Song Jiankang, Löwik Dennis W P M, Seyednejad Hajar, Leeuwenburgh Sander C G
Department of Biomaterials, Radboud University Medical Center, Philips van Leydenlaan 25, 6525 EX Nijmegen, The Netherlands.
FeyeCon Development and Implementation B.V., Rijnkade 17A, 1382 GS Weesp, The Netherlands.
Acta Biomater. 2017 Aug;58:67-79. doi: 10.1016/j.actbio.2017.05.059. Epub 2017 Jun 1.
Multicompartment particles, which are particles composed of smaller building units, have gained considerable interest during the past decade to facilitate simultaneous and differential delivery of several biomolecules in various applications. Supercritical carbon dioxide (CO) processing is an industrial technology widely used for large-scale synthesis and processing of materials. However, the application of this technology for production of multicompartment particles from colloidal particles has not yet been explored. Here, we report the formation of raspberry-like gelatin (RLG) microparticles composed of gelatin nanoparticles as colloidal building blocks through supercritical CO processing. We show that these RLG microparticles exhibit a high stability upon dispersion in aqueous media without requiring chemical cross-linking. We further demonstrate that these microparticles are cytocompatible and facilitate differential release of two different model compounds. The strategy presented here can be utilized as a cost-effective route for production of various types of multicompartment particles using colloidal particles with suitable interparticle interactions.
Multicompartment particles have gained considerable interest during the past decade to facilitate simultaneous and differential delivery of multiple biomolecules in various biomedical applications. Nevertheless, common methods employed for the production of such particles are often complex and only offer small-scale production. Here, we report the formation of raspberry-like gelatin (RLG) microparticles composed of gelatin nanoparticles as colloidal building blocks through supercritical CO processing. We show that these microparticles are cytocompatible and facilitate differential release of two model compounds with different molecular sizes, promising successful applications in various biomedical areas. Summarizing, this paper presents a novel strategy that can be utilized as a cost-effective route for production of various types of multicompartment particles using a wide range of colloidal building blocks.
多隔室颗粒是由较小的构建单元组成的颗粒，在过去十年中受到了广泛关注，可用于在各种应用中同时和差异递送多种生物分子。超临界二氧化碳（CO₂）处理是一种广泛用于材料大规模合成和加工的工业技术。然而，该技术在由胶体颗粒生产多隔室颗粒方面的应用尚未得到探索。在此，我们报告了通过超临界CO₂处理形成了由明胶纳米颗粒作为胶体构建块组成的树莓状明胶（RLG）微粒。我们表明，这些RLG微粒在分散于水性介质中时表现出高稳定性，无需化学交联。我们进一步证明，这些微粒具有细胞相容性，并有助于两种不同模型化合物的差异释放。本文提出的策略可作为一种经济有效的途径，用于使用具有合适颗粒间相互作用的胶体颗粒生产各种类型的多隔室颗粒。
在过去十年中，多隔室颗粒在各种生物医学应用中促进多种生物分子的同时和差异递送方面受到了广泛关注。然而，用于生产此类颗粒的常用方法通常很复杂，且只能进行小规模生产。在此，我们报告了通过超临界CO₂处理形成了由明胶纳米颗粒作为胶体构建块组成的树莓状明胶（RLG）微粒。我们表明，这些微粒具有细胞相容性，并有助于两种不同分子大小的模型化合物的差异释放，有望在各种生物医学领域成功应用。总之，本文提出了一种新颖的策略，可作为一种经济有效的途径，用于使用各种胶体构建块生产各种类型的多隔室颗粒。
