Itoh Harumichi, Nishikawa Shimpei, Haraguchi Tomoya, Arikawa Yu, Hiyama Masato, Eto Shotaro, Iseri Toshie, Itoh Yoshiki, Tani Kenji, Nakaichi Munekazu, Taura Yasuho, Itamoto Kazuhito
Department of Small Animal Clinical Science, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677-1 Yoshida, Yamaguchi-shi, Yamaguchi 753-8515, Japan.
Department of Veterinary Surgery, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677-1 Yoshida, Yamaguchi-shi, Yamaguchi 753-8515, Japan.
J Vet Med Sci. 2017 Sep 12;79(9):1540-1544. doi: 10.1292/jvms.16-0503. Epub 2017 Jun 5.
Adipose-derived stem cells (ADSCs) are abundant and readily obtained, and have been studied for their clinical applicability in regenerative medicine. Some surface antigens have been identified as markers of different ADSC subpopulations in mice and humans. However, it is unclear whether functionally distinct subpopulations exist in dogs. To address this issue, we evaluated aldehyde dehydrogenase (ALDH) activity-a widely used stem cell marker in mice and humans-by flow cytometry. Approximately 20% of bulk ADSCs showed high ALDH activity. Compared to cells with low activity (ALDH), the high-activity (ALDH) subpopulation exhibited a higher capacity for adipogenic and osteogenic differentiation. This is the first report of distinct ADSC subpopulations in dogs that differ in terms of adipogenic and osteogenic differentiation potential.
脂肪来源干细胞（ADSCs）数量丰富且易于获取，人们已对其在再生医学中的临床适用性进行了研究。一些表面抗原已被鉴定为小鼠和人类不同ADSC亚群的标志物。然而，尚不清楚犬类中是否存在功能不同的亚群。为解决这一问题，我们通过流式细胞术评估了醛脱氢酶（ALDH）活性——一种在小鼠和人类中广泛使用的干细胞标志物。大约20%的大量ADSCs显示出高ALDH活性。与低活性（ALDH）细胞相比，高活性（ALDH）亚群表现出更高的成脂和成骨分化能力。这是关于犬类中具有不同成脂和成骨分化潜能的独特ADSC亚群的首次报道。
