用于检测DNA中半甲基化CpG位点的工程亲和剂。
Engineering affinity agents for the detection of hemi-methylated CpG sites in DNA.
作者信息
Tarn B E, Sung K-J, Sikes H D
机构信息
Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA, 02139, USA.
出版信息
Mol Syst Des Eng. 2016 Oct 1;1(3):273-277. doi: 10.1039/C6ME00073H. Epub 2016 Sep 7.
Abstract
Wild-type methyl-CpG-binding domain (MBD) proteins specifically bind symmetrically methylated DNA sequences, and assays have been developed that use these proteins for profiling DNA methylation. Here, we use directed evolution in the yeast surface display format to identify a new protein variant that binds hemi-methylated CpG dinucleotides.
摘要
野生型甲基化CpG结合结构域（MBD）蛋白特异性结合对称甲基化的DNA序列，并且已经开发出利用这些蛋白对DNA甲基化进行分析的方法。在此，我们采用酵母表面展示形式的定向进化来鉴定一种能结合半甲基化CpG二核苷酸的新蛋白变体。
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