Mirhoseini Mehri, Talebpour Amiri Fereshteh, Karimpour Malekshah Abbas Ali, Rezanejad Gatabi Zahra, Ghaffari Elmira
Amol Faculty of Nursing and Midwifery, Mazandaran University of Medical Sciences, Sari, Iran.
Department of Anatomical Sciences, Immunogenetics Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
Int J Reprod Biomed. 2017 Mar;15(3):141-146.
Testicular damage due to spermatic cord torsion may lead to infertility. It is probably because of changes in oxidative stress factors such as malondialdehyde.
To investigate the protective effect of melatonin (MLT), as an antioxidant, on testicular damage induced by acute unilateral spermatic cord torsion and detorsion (T/D) in rats.
In this experimental study, 48 adult male Wistar rats were randomly divided into three groups (8 rats/group): sham group underwent right scrotal surgery only., the T/D group underwent right testicular torsion (for 1 hr) and detorsion, and the melatonin group underwent right testicular torsion, received 25 µg/kg melatonin intraperitoneally immediately after surgery of T/D. Then the histological parameters and malondialdehyde (MDA) changes were evaluated.
Torsion and detorsion decreased the diameter of the tubules significantly compared to controls (p=0.003). Melatonin could increase the diameter, but it was not significant (p=0.26). The heights of the epithelium were constant in sham, T/D, and melatonin groups without any significant difference between groups (p=0.98). Based on Johnsen's score, spermatogenesis was normal in the sham group. The torsion significantly injured all lineage cells (p<0.001). There was no any spermatid or sperm in the seminiferous tubules. Melatonin improved the spermatogenesis significantly (p=0.02), but could not improve MDA level significantly (p=0.99).
Severe degenerative changes of testis were induced by acute unilateral spermatic cord torsion and detorsion in rats, but it had no effect on MDA level.
精索扭转导致的睾丸损伤可能会引起不育。这可能是由于诸如丙二醛等氧化应激因子的变化所致。
研究褪黑素（MLT）作为一种抗氧化剂，对大鼠急性单侧精索扭转和复位（T/D）所致睾丸损伤的保护作用。
在本实验研究中，48只成年雄性Wistar大鼠被随机分为三组（每组8只）：假手术组仅进行右侧阴囊手术；T/D组进行右侧睾丸扭转（持续1小时）然后复位；褪黑素组进行右侧睾丸扭转，在T/D手术后立即腹腔注射25μg/kg褪黑素。然后评估组织学参数和丙二醛（MDA）的变化。
与对照组相比，扭转和复位显著减小了曲细精管的直径（p = 0.003）。褪黑素可增加曲细精管直径，但差异不显著（p = 0.26）。假手术组、T/D组和褪黑素组的上皮高度保持恒定，组间无显著差异（p = 0.98）。根据约翰森评分，假手术组精子发生正常。扭转显著损伤了所有谱系细胞（p < 0.001）。生精小管中没有任何精子细胞或精子。褪黑素显著改善了精子发生（p = 0.02），但对MDA水平的改善不显著（p = 0.99）。
大鼠急性单侧精索扭转和复位可导致睾丸严重退行性变，但对MDA水平无影响。
