Department of Crop Sciences, University of Illinois, Urbana, IL, USA.
Plant Clinic, University of Illinois, Urbana, IL, USA.
Pest Manag Sci. 2017 Nov;73(11):2221-2224. doi: 10.1002/ps.4632. Epub 2017 Aug 7.
Amaranthus palmeri recently has been brought into the Midwestern USA as a contaminant in Conservation Reserve Program seeding mixes. Rapid species screening is required to mitigate the risk of continued species movement.
Markers were developed for A. palmeri-specific nucleotide polymorphisms in the internal transcribed spacer of the ribosomal coding region. A quantitative polymerase chain reaction (qPCR) assay successfully identified A. palmeri from single-plant samples, simulated mixed-plant samples and seed mixtures.
A qPCR assay for distinguishing A. palmeri from 12 other Amaranthus spp. was developed and validated. The assay can consistently detect a single A. palmeri seed when present in a pool of 100 total Amaranthus spp. seeds. © 2017 Society of Chemical Industry.
凤尾鸡冠花最近作为保护储备计划播种混合物中的污染物被引入美国中西部。需要快速的物种筛选来降低物种持续传播的风险。
在核糖体编码区的内转录间隔区开发了针对凤尾鸡冠花特异性核苷酸多态性的标记。定量聚合酶链反应（qPCR）分析成功地从单株样本、模拟混合株样本和种子混合物中鉴定出了凤尾鸡冠花。
开发并验证了一种区分凤尾鸡冠花和其他 12 种苋菜属植物的 qPCR 分析方法。当 100 粒苋菜属植物种子的混合物中存在一粒凤尾鸡冠花种子时，该方法可以持续检测到。 © 2017 化学工业协会。
