Inagaki N, Kamisaki Y, Kiyama H, Horio Y, Tohyama M, Wada H
Brain Res. 1985 Jan 28;325(1-2):336-9. doi: 10.1016/0006-8993(85)90335-x.
The localization of cytosolic (s) or mitochondrial (m) glutamic oxaloacetic transaminase (GOT) was examined in the rat retina by means of an indirect immunofluorescence method using antibodies specific for s- and m-GOT. The m-GOT-like immunoreactive structures were seen on the inner segments of the photoreceptor cells and other outer and inner plexiform layers. These structures were dot-like in appearance. Somas were not labeled. In contrast, s-GOT-like structures were found on the inner segments and inner fibers of the photoreceptor cells, numerous cell somas in the inner nuclear layer (horizontal, amacrine and bipolar cells), and ganglion cell layer (displaced amacrine cells) and inner plexiform layer. The difference in distribution between s- and m-GOT isozymes suggests that they may be useful as markers for glutamatergic and/or aspartinergic neurons.
利用针对胞质型（s）和线粒体型（m）谷草转氨酶（GOT）的特异性抗体，通过间接免疫荧光法检测了大鼠视网膜中胞质型或线粒体型谷草转氨酶（GOT）的定位。在光感受器细胞的内节以及其他外网状层和内网状层中可见m - GOT样免疫反应结构。这些结构外观呈点状。胞体未被标记。相比之下，在光感受器细胞的内节和内纤维、内核层的众多细胞胞体（水平细胞、无长突细胞和双极细胞）、神经节细胞层（移位无长突细胞）和内网状层中发现了s - GOT样结构。s - GOT和m - GOT同工酶在分布上的差异表明，它们可用作谷氨酸能和/或天冬氨酸能神经元的标志物。
