Casavola V, Svelto M
Comp Biochem Physiol A Comp Physiol. 1985;80(3):329-32. doi: 10.1016/0300-9629(85)90045-3.
Treatment of ventral frog skin with serosal A23187 calcium ionophore caused an initially transient increase in transepithelial sodium transport. After 60 min of treatment with A23187, a steady-state transport value was reached which was significantly lower than the initial one. Furthermore, it was found that ionophore treatment greatly inhibited the natriferic response to ADH and to 8br-cAMP. A further analysis on the possible ionophore action mechanism was carried out through pretreatment of the skin with indomethacin, very powerful prostaglandin synthesis inhibitor. In the experimental conditions reported, A23187 seems no longer capable of inducing a transient increase in sodium transport, although it does inhibit the natriferic response to ADH.
用浆膜A23187钙离子载体处理青蛙腹部皮肤，会使跨上皮钠转运最初出现短暂增加。在用A23187处理60分钟后，达到一个稳态转运值，该值显著低于初始值。此外，发现离子载体处理极大地抑制了对抗利尿激素（ADH）和8-溴环磷酸腺苷（8br-cAMP）的促钠反应。通过用吲哚美辛（一种非常有效的前列腺素合成抑制剂）对皮肤进行预处理，对离子载体可能的作用机制进行了进一步分析。在报告的实验条件下，A23187似乎不再能够诱导钠转运的短暂增加，尽管它确实抑制了对ADH的促钠反应。
