Department of Medical Microbiology, 2nd Faculty of Medicine, Charles University in Prague and Motol University Hospital, Czech Republic; DNA Laboratory, Department of Paediatric Neurology, 2nd Faculty of Medicine, Charles University in Prague and Motol University Hospital, Czech Republic.
Department of Medical Microbiology, 2nd Faculty of Medicine, Charles University in Prague and Motol University Hospital, Czech Republic.
J Microbiol Immunol Infect. 2018 Jun;51(3):344-351. doi: 10.1016/j.jmii.2017.02.002. Epub 2017 May 24.
To characterise and compare twenty-eight Finnish Clostridium difficile RT027-like isolates, selected based on the presence of 18 bp deletion in the tcdC gene and toxin gene profile (A, B, binary), with eleven RT027 isolates from different Finnish geographical areas and time periods.
Twenty-eight C. difficile RT027-like isolates and 11 RT027 comparative strains were characterised by capillary-electrophoresis (CE) ribotyping, multi-locus variable tandem-repeats analysis (MLVA), multi-locus sequence typing (MLST), and sequencing of tcdC and gyrA gene fragments. Susceptibility to moxifloxacin was determined by E-test.
Of 28 RT027-like isolates, seven RTs (016, 034, 075, 080, 153, 176 and 328), three WEBRIBO types (411, 475, AI-78) and three new profiles (F1-F3) were identified. MLVA revealed six clonal complexes (RTs 016, 027, 176 and F3). MLST showed eleven sequence types (1, 41, 47, 67, 95, 191,192, 223, 229, 264 and new ST). Twenty-two isolates (RTs 016, 080, 176, 328, F1, F2, F3 and WRTAI-78) carried Δ117 in the tcdC gene. Isolates of RTs 016, 027 and 176 were moxifloxacin resistant and harboured Thr82Ile in the GyrA.
Our results show a high diversity within 28 Finnish RT027-like C. difficile isolates, with twelve CE-ribotyping profiles and eleven STs. MLVA revealed the regional spread of RTs 016, 027, 176 and F3. The presence of Δ117 in the tcdC gene in eight non-027 RTs highlights the importance of careful interpretation of the results from molecular systems targeting this site in the genome of C. difficile and the need of strain typing for epidemiological purposes.
对 28 株基于 tcdC 基因 18bp 缺失和毒素基因谱（A、B、二进制）选择的芬兰产艰难梭菌 RT027 类似株，与来自不同芬兰地理区域和时间的 11 株 RT027 分离株进行特征描述和比较。
对 28 株艰难梭菌 RT027 类似株和 11 株 RT027 对照株进行毛细管电泳（CE）核糖体分型、多位点可变串联重复分析（MLVA）、多位点序列分型（MLST）和 tcdC 和 gyrA 基因片段测序。通过 E 试验测定莫西沙星的敏感性。
28 株 RT027 类似株中，7 株 RT（016、034、075、080、153、176 和 328）、3 株 WEBRIBO 型（411、475、AI-78）和 3 种新图谱（F1-F3）被鉴定。MLVA 显示了 6 个克隆复合体（RTs 016、027、176 和 F3）。MLST 显示了 11 种序列类型（1、41、47、67、95、191、192、223、229、264 和新 ST）。22 株（RTs 016、080、176、328、F1、F2、F3 和 WRTAI-78）在 tcdC 基因中携带 Δ117。RTs 016、027 和 176 的分离株对莫西沙星耐药，gyrA 中存在 Thr82Ile。
我们的结果显示，28 株芬兰产 RT027 艰难梭菌类似株具有高度多样性，有 12 种 CE-核糖体分型谱和 11 种 ST。MLVA 显示了 RTs 016、027、176 和 F3 在地区的传播。在 8 株非 027 RT 中存在 tcdC 基因中的 Δ117，这凸显了在艰难梭菌基因组中仔细解释针对该位点的分子系统结果的重要性，以及为了流行病学目的需要对菌株进行分型。
