Fukushima Nanae, Yokouchi Kumiko, Kuroiwa Masafumi, Kawagishi Kyutaro, Moriizumi Tetsuji
Department of Anatomy, Shinshu University School of Medicine, Matsumoto, Nagano 390-8621, Japan.
Department of Anatomy, Shinshu University School of Medicine, Matsumoto, Nagano 390-8621, Japan.
Neurosci Lett. 2017 Jul 13;653:215-219. doi: 10.1016/j.neulet.2017.06.001. Epub 2017 Jun 3.
It is well known that acetyl-l-carnitine (ALC) has various neuroprotective effects against neurodegenerative diseases. In addition, it has been reported that ALC facilitates myelination of regenerated axons after peripheral nerve injuries. We previously reported that spontaneous regeneration of the lateral olfactory tract (LOT), the main fiber tract of the central olfactory system, consistently occurred in newborn rats and a majority of these regenerated fibers were unmyelinated in neonatally LOT-transected young adult rats. To investigate the effects of ALC treatment on myelination in LOT, neonatal rats were treated with ALC after LOT transection. Immunohistochemistry for myelin basic protein showed more positive areas in ALC-treated rats than in control rats. Moreover, the number of myelinated axons of regenerated fibers was assessed using electron microscopy and was found to be statistically higher in ALC-treated rats compared to control rats. The study revealed that ALC accelerates myelination of regenerated fibers in neonatally LOT-injured young adult rats.
众所周知，乙酰左旋肉碱（ALC）对神经退行性疾病具有多种神经保护作用。此外，有报道称ALC可促进周围神经损伤后再生轴突的髓鞘形成。我们之前报道过，中枢嗅觉系统的主要纤维束——外侧嗅束（LOT）在新生大鼠中会持续发生自发再生，并且在新生期LOT横断的成年幼鼠中，这些再生纤维大多数是无髓鞘的。为了研究ALC治疗对LOT髓鞘形成的影响，在LOT横断后，对新生大鼠进行了ALC治疗。髓鞘碱性蛋白的免疫组织化学显示，与对照大鼠相比，接受ALC治疗的大鼠中有更多的阳性区域。此外，使用电子显微镜评估再生纤维的有髓轴突数量，发现与对照大鼠相比，接受ALC治疗的大鼠中有髓轴突数量在统计学上更高。该研究表明，ALC可加速新生期LOT损伤的成年幼鼠中再生纤维的髓鞘形成。
