Regazzi F M, Silva L C G, Lúcio C F, Veiga G A L, Angrimani D S R, Kishi D, Barbosa M M M, Vannucchi C I
Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, Rua Prof. Orlando Marques de Paiva, 87 - Cidade Universitária, 05508-270, São Paulo, Brazil.
Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, Rua Prof. Orlando Marques de Paiva, 87 - Cidade Universitária, 05508-270, São Paulo, Brazil.
Theriogenology. 2017 Jul 15;97:179-185. doi: 10.1016/j.theriogenology.2017.04.039. Epub 2017 Apr 27.
Although the effects of antenatal corticosteroid therapy in clinical improvement and pulmonary maturation in preterm have been described, little is known on premature newborn puppies. This study aimed to evaluate the effect of maternal administration of a single dose of prenatal betamethasone on lung function of preterm newborn puppies in the first hours of life, especially from the clinical point of view and acid-base balance. A prospective study was conducted involving 21 puppies allocated into three experimental groups: Term Group (63 days post-ovulation), Preterm-Treated Group (57 days post-ovulation and maternal administration of a single dose of 0.5 mg/kg of betamethasone) and Preterm-Control Group (57 days post-ovulation). Puppies were analyzed clinically through the Apgar score, heart rate, respiratory rate and neurological tests (muscular tone and irritability reflex) and for oximetry and blood acid-base balance in distinct experimental moments. Premature puppies had marked degree of prematurity, reversed by maternal administration of betamethasone. Prenatal corticosteroid therapy promoted better pulmonary and metabolic condition, with more efficient compensatory response to acid-base imbalance and better pulmonary gas exchange capacity. Therefore, prenatal treatment with betamethasone can be adopted as clinical lung maturation protocol for pregnancies at risk in order to prevent low vitality and increase neonatal survival.
尽管产前皮质类固醇疗法对早产儿临床状况改善和肺成熟的作用已有描述，但对于早产新生幼犬的情况却知之甚少。本研究旨在评估母犬单次产前注射倍他米松对早产新生幼犬出生后最初几小时肺功能的影响，特别是从临床角度和酸碱平衡方面进行评估。开展了一项前瞻性研究，将21只幼犬分为三个实验组：足月组（排卵后63天）、早产治疗组（排卵后57天且母犬单次注射0.5毫克/千克倍他米松）和早产对照组（排卵后57天）。在不同实验阶段，通过阿氏评分、心率、呼吸频率和神经学检查（肌张力和应激反射）对幼犬进行临床分析，并进行血氧测定和血液酸碱平衡分析。早产幼犬有明显的早产程度，母犬注射倍他米松可使其得到改善。产前皮质类固醇疗法可促进更好的肺部和代谢状况，对酸碱失衡有更有效的代偿反应以及更好的肺气体交换能力。因此，产前使用倍他米松治疗可作为有风险妊娠的临床肺成熟方案，以预防新生儿活力低下并提高新生儿存活率。
