Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, Rua Prof. Orlando Marques de Paiva, 87 - Cidade Universitária, 05508-270, São Paulo, Brazil.
Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, Rua Prof. Orlando Marques de Paiva, 87 - Cidade Universitária, 05508-270, São Paulo, Brazil.
Theriogenology. 2020 Sep 1;153:19-26. doi: 10.1016/j.theriogenology.2020.05.005. Epub 2020 May 7.
Among prematurity complications, the most important disorder is structural immaturity and inadequate production of pulmonary surfactant. Betamethasone is the drug of choice to artificially improve pulmonary capacity, thus we aimed to verify the effect of prenatal maternal treatment on lung development of premature puppies. Pregnant bitches were allocated in Term Group (n = 7), Preterm-Treated Group (interrupted pregnancies with maternal administration of betamethasone; n = 7), Preterm-Control Group (untreated interrupted pregnancies; n = 7), Extremely-Preterm Group (interrupted pregnancies at 55d; n = 6). Puppies were subjected to chest radiographic at birth, morphometric description of pulmonary structures and immunohistochemical analysis of surfactant protein B, proliferating cell nuclear antigen and cytokeratin were performed. In Preterm-Treated Group it was possible to more clearly identify cardiac silhouette and lung parenchyma by X-Ray. Saccular formation was higher in Preterm Groups, while Term Group had higher subsaccular development. Lung septation was higher in Treated and Term Groups. Term Group had higher number of cells marked for SP-B, whereas higher proliferation was observed in Extreme-Preterm and Preterm-Control Groups. Preterm Treated and Term Groups had higher tissue differentiation. In conclusion, antenatal maternal corticotherapy in dogs acted by increasing lung morphology and development of areas of gas exchange, regulate metabolism of pulmonary fluids rather than stimulate surfactant production.
在早产并发症中，最重要的是结构不成熟和肺表面活性物质产生不足。倍他米松是人为改善肺功能的首选药物，因此，我们旨在验证产前母体治疗对早产幼犬肺发育的影响。将怀孕母犬分配到足月组（n=7）、早产治疗组（妊娠中断并给予母马倍他米松；n=7）、早产对照组（未治疗的妊娠中断；n=7）、超早产组（妊娠 55 天中断；n=6）。幼犬在出生时进行胸部 X 光检查，对肺结构进行形态描述，并进行肺表面活性蛋白 B、增殖细胞核抗原和细胞角蛋白的免疫组织化学分析。在早产治疗组中，X 射线可以更清楚地识别心脏轮廓和肺实质。囊泡形成在早产组中较高，而足月组则具有更高的亚囊泡发育。肺间隔在治疗组和足月组中较高。足月组中标记有 SP-B 的细胞数量较多，而超早产组和早产对照组的增殖较高。早产治疗组和足月组的组织分化较高。总之，犬的产前皮质激素治疗通过增加肺形态和气体交换区域的发育来发挥作用，调节肺液的代谢，而不是刺激表面活性物质的产生。
