通过单一四环素诱导慢病毒系统表达大鼠甘丙肽的hTERT永生化骨髓间充质基质细胞
hTERT-Immortalized Bone Mesenchymal Stromal Cells Expressing Rat Galanin via a Single Tetracycline-Inducible Lentivirus System.
作者信息
An Ke, Liu Hui-Ping, Zhong Xiao-Long, Deng David Y B, Zhang Jing-Jun, Liu Zhi-Heng
机构信息
Department of Anesthesiology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China.
Department of Anesthesiology, The Third Xiangya Hospital of Central South University, Changsha 410013, China.
出版信息
Stem Cells Int. 2017;2017:6082684. doi: 10.1155/2017/6082684. Epub 2017 May 11.
The use of human telomerase reverse transcriptase-immortalized bone marrow mesenchymal stromal cells (hTERT-BMSCs) as vehicles to deliver antinociceptive galanin (GAL) molecules into pain-processing centers represents a novel cell therapy strategy for pain management. Here, an hTERT-BMSCs/Tet-on/GAL cell line was constructed using a single Tet-on-inducible lentivirus system, and subsequent experiments demonstrated that the secretion of rat GAL from hTERT-BMSCs/Tet-on/GAL was switched on and off under the control of an inducer in a dose-dependent manner. The construction of this cell line is the first promising step in the regulation of GAL secretion from hTERT-immortalized BMSCs, and the potential application of this system may provide a stem cell-based research platform for pain.
使用人端粒酶逆转录酶永生化骨髓间充质基质细胞（hTERT-BMSCs）作为载体，将具有镇痛作用的甘丙肽（GAL）分子传递到疼痛处理中枢，代表了一种用于疼痛管理的新型细胞治疗策略。在此，使用单一的Tet-on诱导型慢病毒系统构建了hTERT-BMSCs/Tet-on/GAL细胞系，随后的实验表明，hTERT-BMSCs/Tet-on/GAL分泌大鼠GAL的过程在诱导剂的控制下以剂量依赖的方式开启和关闭。该细胞系的构建是调控hTERT永生化BMSCs分泌GAL的第一个有前景的步骤，该系统的潜在应用可能为疼痛研究提供一个基于干细胞的平台。
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