Lee Jae-Hong, Kang Won Kyung, Seo Jae-Hyun, Choi Mi-Yung, Lee Yang Hyun, Kim Hyo Min, Park Kyoung-Ho
Department of Otolaryngology-HNS, The Catholic University of Korea College of Medicine, Seoul, Korea.
Department of Surgery, The Catholic University of Korea College of Medicine, Seoul, Korea.
Korean J Audiol. 2012 Sep;16(2):47-53. doi: 10.7874/kja.2012.16.2.47. Epub 2012 Sep 20.
Regeneration or replacement of damaged hair cells and neurons in the cochlea might be an ideal treatment for sensorineural hearing loss (SNHL). The purpose of the present study was to investigate whether mesenchymal stem cells (MSCs), derived from the bone marrow of rats, could differentiate into auditory hair cells and neurons.
The centrifuge gradient method was used to isolate MSCs from the bone marrow of rats. To confirm whether bone marrow-derived MSCs can differentiate into neuronal cells, culture medium with glial cell-derived neurotrophic factor, brain-derived neurotrophic factor and neurotrophin-3 for 14 days. In addition, immunofluorescence staining and RT-PCR were performed for characterization of the neurospheres and differentiated cells from 7 and 14 day cultures.
The results showed that MSCs could differentiate into neuron-positive and hair cell-positive cells, using different compositions of growth factors. And RT-PCR result was identified high or low of gene expression all these differentiated cells.
Rat bone marrow-derived MSCs differentiated into neuronal progenitor cells. These cells might be useful for the treatment of SNHL.
耳蜗中受损毛细胞和神经元的再生或替代可能是感音神经性听力损失（SNHL）的理想治疗方法。本研究的目的是探讨源自大鼠骨髓的间充质干细胞（MSC）是否能分化为听觉毛细胞和神经元。
采用离心梯度法从大鼠骨髓中分离MSC。为了确认骨髓来源的MSC是否能分化为神经元细胞，将其置于含有胶质细胞源性神经营养因子、脑源性神经营养因子和神经营养素-3的培养基中培养14天。此外，对培养7天和14天的神经球和分化细胞进行免疫荧光染色和逆转录聚合酶链反应（RT-PCR）以进行表征。
结果表明，使用不同组成的生长因子，MSC可分化为神经元阳性和毛细胞阳性细胞。RT-PCR结果确定了所有这些分化细胞基因表达的高低。
大鼠骨髓来源的MSC分化为神经元祖细胞。这些细胞可能对SNHL的治疗有用。
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