Woo Sang Mi, Kwon Sang-Chul, Ko Seong Gyu, Cho Sung-Gook
Department of Science in Korean Medicine, Graduate School of Kyung Hee University, Seoul 130-701, Republic of Korea.
Department of Food Technology, Korea National University of Transportation, Jeungpyeong, Chungbuk 368-701, Republic of Korea.
Biomed Rep. 2017 Jun;6(6):681-685. doi: 10.3892/br.2017.897. Epub 2017 May 3.
Cancer remains a leading cause of mortality worldwide, therefore food products are being investigated for potential prevention or treatment strategies. The ingredient, barley grass extract ( L.; Bex) is used to prevent or ameliorate various types of disease. In cancer, Bex has been revealed to inhibit tumor growth. However, its effect on cancer cells is yet to be clearly defined. In the present study, the effect of Bex on cancer cell growth was investigated. Bex inhibited the viabilities of breast and prostate cancer cells according to the results of MTT assays. Accordingly, Bex caused apoptosis, which was confirmed by Annexin V staining and western blot analysis for poly (ADP-ribose) polymerase and caspases. Furthermore, Bex increased the intracellular levels of reactive oxygen species (ROS), and -acetyl-L-cystein blocked Bex-induced apoptosis. Therefore, the study demonstrated that Bex causes apoptosis of breast and prostate cancer cells by increasing intracellular ROS levels.
癌症仍然是全球主要的死亡原因，因此人们正在研究食品以寻找潜在的预防或治疗策略。成分大麦草提取物（L.；Bex）被用于预防或改善各种类型的疾病。在癌症方面，已发现Bex可抑制肿瘤生长。然而，其对癌细胞的作用尚未明确界定。在本研究中，对Bex对癌细胞生长的影响进行了研究。根据MTT试验结果，Bex抑制了乳腺癌和前列腺癌细胞的活力。相应地，Bex导致细胞凋亡，这通过膜联蛋白V染色以及聚（ADP - 核糖）聚合酶和半胱天冬酶的蛋白质印迹分析得到证实。此外，Bex增加了细胞内活性氧（ROS）水平，并且N - 乙酰 - L - 半胱氨酸可阻断Bex诱导的细胞凋亡。因此，该研究表明Bex通过增加细胞内ROS水平导致乳腺癌和前列腺癌细胞凋亡。
