Kryczyk Agata, Piotrowska Joanna, Sito Magdalena, Sulkowska-Ziaja Katarzyna, Dobosz Konrad, Opoka Włodzimierz, Muszyńska Bożena
a Department of Inorganic and Analytical Chemistry, Faculty of Pharmacy , Jagiellonian University Medical College , Kraków , Poland.
b Department of Pharmaceutical Botany, Faculty of Pharmacy , Jagiellonian University Medical College , Kraków , Poland.
J Environ Sci Health B. 2017 Sep 2;52(9):617-622. doi: 10.1080/03601234.2017.1330068. Epub 2017 Jun 6.
The goal of this study was to evaluate cadmium and lead accumulation ability of in vitro cultures biomass containing selected edible mushroom species derived from the environment (Laetiporus sulphureus, Imleria badia) and those of commercial origin (Agaricus bisporus). Atomic absorption spectrometry was used to evaluate the content of Cd(II) and Pb(II) on the medium supplemented with Cd(II) or Pb(II), each of them at the same concentration of 5·10 M. The highest concentration of Cd(II) ions was determined in the biomass from L. sulphureus in vitro cultures, while the highest concentration of Pb(II) ions was found in the biomass from A. bisporus in vitro cultures. The greatest Cd(II) and Pb(II) accumulation ability in mycelium per dry weight was shown for L. sulphureus. Among the test species, biomass of A. bisporus showed the lowest ability for the bioaccumulation of Cd(II); however, comparable ability for the remediation of Pb(II) was provided by the biomasses from A. bisporus and I. badia in vitro cultures. The results confirm the possibility of using these mushroom species for remediation and indicate the relationship between bioaccumulation of heavy metals and the test species.
本研究的目的是评估源自环境的特定可食用蘑菇物种（硫黄多孔菌、美味牛肝菌）以及商业来源的蘑菇物种（双孢蘑菇）的体外培养生物量对镉和铅的积累能力。采用原子吸收光谱法评估添加了镉（II）或铅（II）的培养基中镉（II）和铅（II）的含量，它们的浓度均为5·10⁻⁶ M。在硫黄多孔菌体外培养生物量中测定出最高浓度的镉（II）离子，而在双孢蘑菇体外培养生物量中发现了最高浓度的铅（II）离子。按干重计，硫黄多孔菌的菌丝体对镉（II）和铅（II）的积累能力最强。在受试物种中，双孢蘑菇的生物量对镉（II）的生物积累能力最低；然而，双孢蘑菇和美味牛肝菌体外培养生物量对铅（II）的修复能力相当。研究结果证实了利用这些蘑菇物种进行修复的可能性，并表明了重金属生物积累与受试物种之间的关系。
