Department of Inorganic and Analytical Chemistry, Faculty of Pharmacy, Jagiellonian University Medical College, 9 Medyczna Street, 30-688 Kraków, Poland.
Department of Analytical Chemistry and Biochemistry, Faculty of Materials Science and Ceramics, AGH University of Science and Technology, Adama Mickiewicza Ave. 30, 30-059 Kraków, Poland.
Molecules. 2021 Aug 26;26(17):5165. doi: 10.3390/molecules26175165.
(J.E. Lange) Imbach is one the most popular species of edible mushrooms in the world because of its taste and nutritional properties. In the research, repeatability of accumulation of bioelements and biomass yield in experimentally chosen in vitro culture medium, was confirmed. The in vitro cultures were conducted on the modified Oddoux medium enriched with bioelements (Mg, Zn, Cu, Fe). The aim of the study was to create an effective method of sampling, which enabled non-invasive monitoring of metals concentrations changes in the medium, during increase of biomass in in vitro cultures. The first, indirect method of sampling was applied. The non-invasive probe (a dipper) for in vitro culture was used; hence, the highest biomass increase and metals accumulation were gained. The method also guaranteed culture sterility. The second method, a direct one, interfered the in vitro culture conditions and growth of mycelium, and as a consequence the lower biomass increase and metals' accumulation were observed. Few cases of contaminations of mycelium in in vitro cultures were observed. The proposed method of non-invasive sampling of the medium can be used to monitor changes in the concentrations of metals in the medium and their accumulation in the mycelium in natural environment. Changes in concentrations of the selected metals over time, determined by the method of atomic absorption spectrometry, made it possible to correlate the obtained results with the specific stages of mycelium development and to attempt to explain the mechanism of sampling metals from the liquid substrate.
(让·埃·朗格)因口感和营养特性，茵巴豪（Imbach）菌成为世界上最受欢迎的食用蘑菇品种之一。在研究中，证实了在实验选择的体外培养基中生物元素积累的可重复性和生物量产量。体外培养是在富含有生物元素（Mg、Zn、Cu、Fe）的改良 Oddoux 培养基上进行的。研究的目的是创建一种有效的采样方法，使人们能够在体外培养过程中生物量增加的同时，对培养基中金属浓度的变化进行非侵入式监测。采用了第一种间接采样方法。使用了用于体外培养的非侵入式探头（勺子），从而获得了最高的生物量增加和金属积累。该方法还保证了培养的无菌性。第二种方法是直接采样方法，干扰了体外培养条件和菌丝的生长，因此观察到生物量增加和金属积累较低。在体外培养中观察到少数几次菌丝污染的情况。所提出的非侵入式培养基采样方法可用于监测自然环境中培养基中金属浓度的变化及其在菌丝中的积累。通过原子吸收光谱法确定的选定金属随时间的浓度变化，使我们能够将获得的结果与菌丝发育的特定阶段相关联，并尝试解释从液体基质中采样金属的机制。
