Stanga Paulo Eduardo, Pastor-Idoate Salvador, Zambrano Isaac, Carlin Paul, McLeod David
Manchester Royal Eye Hospital, Central Manchester University Hospitals NHS Foundation Trust, Manchester Academic Health Science Centre, Manchester, United Kingdom.
Manchester Vision Regeneration (MVR) Lab at Manchester Royal Eye Hospital and NIHR/ Wellcome Trust Manchester CRF, Manchester, United Kingdom.
PLoS One. 2017 Jun 6;12(6):e0178462. doi: 10.1371/journal.pone.0178462. eCollection 2017.
To evaluate porcine vitreous flow and water flow rates in a new prototype hypersonic vitrectomy system compared to currently available pneumatic guillotine vitrectors (GVs) systems.
Two vitrectors were tested, a prototype, ultrasound-powered, hypersonic vitrector (HV) and a GV. Porcine vitreous was obtained within 12 to 24 h of sacrifice and kept at 4°C. A vial of vitreous or water was placed on a precision balance and its weight measured before and after the use of each vitrector. Test parameters included changes in aspiration levels, vitrector gauge, cut rates for GVs, % ultrasound (US) power for HVs, and port size for HVs. Data was analysed using linear regression and t-tests.
There was no difference in the total average mean water flow between the 25-gauge GV and the 25-gauge HV (t-test: P = 0.363); however, 25-gauge GV was superior (t-test: P < 0.001) in vitreous flow. The 23-gauge GV was only more efficient in water and vitreous removal than 23-gauge HV needle-1 (Port 0.0055) (t-test: P < 0.001). For HV, wall thickness and gauge had no effect on flow rates. Water and vitreous flows showed a direct correlation with increasing aspiration levels and % US power (p<0.05).
The HV produced consistent water and vitreous flow rates across the range of US power and aspiration levels tested. Hypersonic vitrectomy may be a promising new alternative to the currently available guillotine-based technologies.
与目前可用的气动断头台式玻璃体切割器(GV)系统相比,评估新型原型高超声速玻璃体切割系统中猪玻璃体流速和水流速。
测试了两种玻璃体切割器,一种原型超声驱动的高超声速玻璃体切割器(HV)和一种GV。在处死后12至24小时内获取猪玻璃体,并保存在4°C。将一小瓶玻璃体或水放在精密天平上,在使用每种玻璃体切割器之前和之后测量其重量。测试参数包括抽吸水平的变化、玻璃体切割器规格、GV的切割速率、HV的超声(US)功率百分比以及HV的端口尺寸。使用线性回归和t检验分析数据。
25号GV和25号HV之间的总平均水流没有差异(t检验:P = 0.363);然而,25号GV在玻璃体流速方面更具优势(t检验:P < 0.001)。23号GV在水和玻璃体清除方面仅比23号HV针-1(端口0.0055)更有效(t检验:P < 0.001)。对于HV,壁厚和规格对流速没有影响。水和玻璃体流速与抽吸水平和US功率百分比的增加呈直接相关(p<0.05)。
在测试的US功率和抽吸水平范围内,HV产生了一致的水和玻璃体流速。高超声速玻璃体切割术可能是目前基于断头台技术的一种有前景的新替代方法。