Kong Hui, Wang Xueqian, Shi Rongfeng, Zhao Yan, Cheng Jinjun, Yan Xin, Liu Xiaoman, Wang Yongzhi, Zhang Meiling, Wang Qingguo, Qu Huihua
School of Chinese Medicine, Beijing Key Laboratory, Beijing University of Chinese Medicine, Beijing 100029, China.
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China.
Molecules. 2017 Jun 5;22(6):939. doi: 10.3390/molecules22060939.
Puerarin (PUE) is a compound isolated from the roots of . We studied the pharmacokinetics and tissue distribution kinetics of PUE in Sprague-Dawley rats following intraperitoneal administration of three concentrations. Indirect competitive ELISA based on an anti-PUE monoclonal antibody was used to determine the concentration of PUE in the blood, heart, liver, spleen, lung, kidney, hippocampus, cerebral cortex, and striatum. The plasma and tissue distribution kinetic characteristics following a single injection of PUE (20, 40 and 80 mg/kg) were calculated using a non-compartment model. In the high-dose (80 mg/kg) and medium-dose (40 mg/kg) groups, the kinetic profile of PUE in blood and kidney samples showed two absorption peaks, while that of the other tissues showed only one peak. In the low-dose (20 mg/kg) group, there was only one peak, irrespective of the sample type. Pharmacokinetic parameters, such as the area under the curve, C, and T varied according to the administered dose. AUC and C values increased dose-dependently. PUE was widely distributed in areas of the brain such as the hippocampus, cerebral cortex, and striatum, providing a foundation for guiding the use of PUE in the treatment of cerebral ischaemic stroke and neurodegenerative diseases.
葛根素（PUE）是从[植物名称]根中分离出的一种化合物。我们研究了腹腔注射三种浓度的PUE后，其在Sprague-Dawley大鼠体内的药代动力学和组织分布动力学。基于抗PUE单克隆抗体的间接竞争ELISA法用于测定血液、心脏、肝脏、脾脏、肺脏、肾脏、海马体、大脑皮层和纹状体中PUE的浓度。采用非房室模型计算单次注射PUE（20、40和80 mg/kg）后的血浆和组织分布动力学特征。在高剂量（80 mg/kg）和中剂量（40 mg/kg）组中，血液和肾脏样本中PUE的动力学曲线显示有两个吸收峰，而其他组织仅显示一个峰。在低剂量（20 mg/kg）组中，无论样本类型如何，均只有一个峰。药代动力学参数，如曲线下面积、C和T，随给药剂量而变化。AUC和C值呈剂量依赖性增加。PUE广泛分布于大脑的海马体、大脑皮层和纹状体等区域，为指导PUE用于治疗脑缺血性中风和神经退行性疾病奠定了基础。
