Department of Biosciences and Biotechnology, Fukui Prefectural University, Fukui, Japan.
PLoS One. 2021 Apr 22;16(4):e0249367. doi: 10.1371/journal.pone.0249367. eCollection 2021.
Dermal fibroblast aging contributes to aging-associated functional defects in the skin since dermal fibroblasts maintain skin homeostasis by interacting with the epidermis and extracellular matrix. Here, we found that puerarin, an isoflavone present in Pueraria lobata (Kudzu), can prevent the development of the aging-phenotype in human dermal fibroblasts. Normal human dermal fibroblasts (NHDFs) were subcultivated and high-passage cells were selected as senescent cells, whereas low-passage cells were selected as a young cell control. Puerarin treatment increased cell proliferation and decreased the proportion of senescence-associated beta-galactosidase-positive cells in a high-passage culture of NHDFs. Moreover, puerarin treatment reduced the number of smooth muscle actin (SMA)-positive myofibroblasts and the expression of a reticular fibroblast marker, calponin 1 (CNN1), which were induced in high-passage NHDFs. Fulvestrant, an estrogen receptor antagonist, blocked the puerarin-mediated downregulation of SMA and CNN1. Our results suggest that puerarin may be a useful functional food that alleviates aging-related functional defects in dermal fibroblasts.
皮肤成纤维细胞老化导致与衰老相关的皮肤功能缺陷，因为皮肤成纤维细胞通过与表皮和细胞外基质相互作用来维持皮肤稳态。在这里，我们发现葛根素，一种存在于葛根（葛）中的异黄酮，可以预防人皮肤成纤维细胞衰老表型的发展。正常的人皮肤成纤维细胞（NHDFs）被传代培养，高传代细胞被选为衰老细胞，而低传代细胞被选为年轻细胞对照。葛根素处理增加了细胞增殖，并降低了高传代 NHDFs 中衰老相关的β-半乳糖苷酶阳性细胞的比例。此外，葛根素处理减少了平滑肌肌动蛋白（SMA）阳性肌成纤维细胞的数量和网状纤维标记物钙调蛋白 1（CNN1）的表达，这些在高传代 NHDFs 中被诱导。他莫昔芬，一种雌激素受体拮抗剂，阻断了葛根素介导的 SMA 和 CNN1 的下调。我们的结果表明，葛根素可能是一种有用的功能性食品，可以缓解皮肤成纤维细胞与衰老相关的功能缺陷。
