Department of Anesthesiology and Critical Care Medicine, Jichi Medical University School of Medicine, 3311-1 Yakushiji, Shimotsuke, Tochigi 329-0498, Japan.
Department of Orthopaedic Surgery, Surgical Science, Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa 259-1193, Japan.
Biomaterials. 2014 Feb;35(7):2199-206. doi: 10.1016/j.biomaterials.2013.11.071. Epub 2013 Dec 20.
In our previous studies, we have demonstrated effective regeneration of cartilage through the creation and application of layered cell sheets that combine both chondrocytes and synovial cells. In this study, we were able to demonstrate that cells derived from cell sheets can survive for long periods after transplantation into rat knee joints having osteochondral defects. We established a method for generating cell sheets from firefly luciferase-expressing chondrocytes obtained from transgenic Lewis rats, and carried out allogenic transplantation of these cell sheets into wild-type Lewis rats. We then administered luciferin and monitored the survival of the transplanted cells by using bioluminescence imaging (BLI). Our data showed that the transplanted cells survived and could be detected for more than 21 months, which was longer than expected. Furthermore, the BLI data showed that the transplanted cells remained in the knee joint and did not migrate to other parts of the body, thus confirming the safety of the cell sheets. In this study, we monitored the duration of survival of cell sheets composed of only chondrocytes, only synovial cells, or both chondrocytes and synovial cells, and found that all three types of cell sheets survived for an extended period of time.
在我们之前的研究中，我们通过创建和应用结合软骨细胞和滑膜细胞的层状细胞片，成功实现了软骨的有效再生。在这项研究中，我们能够证明源自细胞片的细胞在移植到具有骨软骨缺损的大鼠膝关节后可以长期存活。我们建立了一种从转荧光素酶表达软骨细胞的转基因 Lewis 大鼠中生成细胞片的方法，并将这些细胞片同种异体移植到野生型 Lewis 大鼠中。然后，我们给予荧光素，并通过生物发光成像 (BLI) 监测移植细胞的存活情况。我们的数据表明，移植细胞存活并可检测超过 21 个月，这比预期的时间要长。此外，BLI 数据表明，移植细胞留在膝关节内，不会迁移到身体的其他部位，从而证实了细胞片的安全性。在这项研究中，我们监测了仅由软骨细胞、仅滑膜细胞或软骨细胞和滑膜细胞组成的细胞片的存活时间，发现所有三种类型的细胞片都能存活很长时间。
