Nikitaki Zacharenia, Pavlopoulou Athanasia, Holá Marcela, Donà Mattia, Michalopoulos Ioannis, Balestrazzi Alma, Angelis Karel J, Georgakilas Alexandros G
DNA Damage Laboratory, Physics Department, School of Applied Mathematical and Physical Sciences, National Technical University of Athens, Iroon Polytechniou 9, 15780 Zografou, Greece.
Department of Computer Science and Biomedical Informatics, University of Thessaly, Papasiopoulou 2-4, 35100 Lamia, Greece.
Cancers (Basel). 2017 Jun 6;9(6):65. doi: 10.3390/cancers9060065.
The mechanisms of response to radiation exposure are conserved in plants and animals. The DNA damage response (DDR) pathways are the predominant molecular pathways activated upon exposure to radiation, both in plants and animals. The conserved features of DDR in plants and animals might facilitate interdisciplinary studies that cross traditional boundaries between animal and plant biology in order to expand the collection of biomarkers currently used for radiation exposure monitoring (REM) in environmental and biomedical settings. Genes implicated in trans-kingdom conserved DDR networks often triggered by ionizing radiation (IR) and UV light are deposited into biological databases. In this study, we have applied an innovative approach utilizing data pertinent to plant and human genes from publicly available databases towards the design of a 'plant radiation biodosimeter', that is, a plant and DDR gene-based platform that could serve as a REM reliable biomarker for assessing environmental radiation exposure and associated risk. From our analysis, in addition to REM biomarkers, a significant number of genes, both in human and not yet characterized as DDR, are suggested as possible DNA repair players. Last but not least, we provide an example on the applicability of an based plant system monitoring the role of cancer-related DNA repair genes , and in processing DNA lesions.
植物和动物对辐射暴露的反应机制是保守的。DNA损伤反应（DDR）途径是植物和动物暴露于辐射后激活的主要分子途径。植物和动物DDR的保守特征可能有助于跨学科研究，跨越动物生物学和植物生物学之间的传统界限，以扩大目前在环境和生物医学环境中用于辐射暴露监测（REM）的生物标志物的收集范围。涉及跨王国保守DDR网络的基因通常由电离辐射（IR）和紫外线触发，并已存入生物数据库。在本研究中，我们应用了一种创新方法，利用来自公开数据库的与植物和人类基因相关的数据，设计了一个“植物辐射生物剂量计”，即一个基于植物和DDR基因的平台，它可以作为一个可靠的REM生物标志物，用于评估环境辐射暴露和相关风险。通过我们的分析，除了REM生物标志物外，大量在人类中尚未被鉴定为DDR的基因被认为可能是DNA修复参与者。最后但同样重要的是，我们提供了一个基于植物系统的适用性示例，该系统监测癌症相关DNA修复基因 、 和 在处理DNA损伤中的作用。
