Liu Xiaohong, Sun Jiamin, Wu Haizhen
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China; Shanghai Engineering Research Center of Maricultured Animal Vaccines, Shanghai 200237, China.
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
Vaccine. 2017 Jul 5;35(31):3813-3816. doi: 10.1016/j.vaccine.2017.05.066. Epub 2017 Jun 3.
Reverse vaccinology (RV) has become a popular method for developing vaccines. Although Edwardsiella tarda is deemed to be an important fish pathogen, so far, no reports have used a genome-based approach to screen vaccine candidates against E. tarda. In the current study, protective antigens of E. tarda were screened using RV. Large-scale cloning, expression and purification of potential candidates were carried out, and their immunoprotective potential was evaluated. A candidate fructose-bisphosphate aldolase (FBA) exhibited broad spectrum protection, as did another glycolysis-related protein glyceraldehyde-3-phosphate dehydrogenase (GAPDH), which we reported previously, indicating the potential of other glycolysis-related proteins of E. tarda as broad spectrum protective antigens. In total, half (5 out 10) of these proteins showed prominent immunoprotective potential. Therefore, we suggest that glycolysis-related proteins are a class of potential broad spectrum protective antigens and that these proteins should be preferentially selected.
反向疫苗学（RV）已成为开发疫苗的一种常用方法。尽管迟缓爱德华氏菌被认为是一种重要的鱼类病原体，但迄今为止，尚无使用基于基因组的方法筛选抗迟缓爱德华氏菌疫苗候选物的报道。在本研究中，利用反向疫苗学筛选了迟缓爱德华氏菌的保护性抗原。对潜在候选物进行了大规模克隆、表达和纯化，并评估了它们的免疫保护潜力。一种候选果糖二磷酸醛缩酶（FBA）表现出广谱保护作用，我们之前报道的另一种与糖酵解相关的蛋白甘油醛-3-磷酸脱氢酶（GAPDH）也是如此，这表明迟缓爱德华氏菌的其他与糖酵解相关的蛋白具有作为广谱保护性抗原的潜力。这些蛋白中总共有一半（10个中的5个）显示出显著的免疫保护潜力。因此，我们认为与糖酵解相关的蛋白是一类潜在的广谱保护性抗原，应优先选择这些蛋白。
