Ma Ke, Deng Lei, Wu Yuanjie, Gao Yuan, Fan Jianhua, Wu Haizhen
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
Department of Applied Biology, East China University of Science and Technology, Shanghai 200237, China.
Mar Drugs. 2024 Dec 12;22(12):555. doi: 10.3390/md22120555.
SR21, a kind of eukaryotic heterotrophic organism rich in unsaturated fatty acids, is an emerging microbial alternative to fish oil. The dietary inclusion of 15% SR21 was optimal for the growth performance of zebrafish. Previous studies demonstrated that fructose-1,6-bisphosphate aldolase (FBA) of is a valuable broad-spectrum antigen against various pathogens in aquaculture (e.g., , , , ). We pioneered the development of stable SR21 transformants expressing the antigen protein FBA, exploring their potential as a novel oral vaccine for the aquaculture industry. The model animal zebrafish () and ornamental fish koi carp ( koi) were harnessed to assess the immunoprotective effect, respectively. According to the quantitative expression analysis, zebrafish fed with recombinant expressing FBA exhibited specific immune responses in the intestine. The expression levels of and , involved in cell-mediated adaptive immune responses, were significantly upregulated on the 14th and 28th days post-immunization. Additionally, the expression of highly specialized antibody genes and in mucosal immunity were significantly triggered on the 14th day post-immunization. Feeding koi carp with recombinant SR21-FBA increased the production of myeloperoxidase and FBA-specific antibodies in the sera. Furthermore, the sera of koi fed with recombinant SR21-FBA exhibited significant bactericidal activities against pathogen . Thus, SR21 is a natural and highly promising oral vaccine carrier that not only provides essential nutrients as a functional feed ingredient but also offers specific immune protection to aquatic animals. This dual application is vital for promoting the sustainable development of the aquaculture industry.
SR21是一种富含不饱和脂肪酸的真核异养生物，是一种新兴的鱼油微生物替代品。日粮中添加15%的SR21对斑马鱼的生长性能最为适宜。先前的研究表明，[具体生物名称]的果糖-1,6-二磷酸醛缩酶（FBA）是一种针对水产养殖中各种病原体（如[列举的病原体名称]）的有价值的广谱抗原。我们率先开发了表达抗原蛋白FBA的稳定SR21转化体，探索它们作为水产养殖业新型口服疫苗的潜力。分别利用模式动物斑马鱼（[具体品种名称]）和观赏鱼锦鲤（锦鲤）来评估免疫保护效果。根据定量表达分析，喂食表达FBA的重组[具体名称]的斑马鱼在肠道中表现出特异性免疫反应。参与细胞介导的适应性免疫反应的[相关基因名称1]和[相关基因名称2]的表达水平在免疫后第14天和第28天显著上调。此外，黏膜免疫中高度特异性抗体基因[抗体基因名称1]和[抗体基因名称2]的表达在免疫后第14天被显著激活。用重组SR21-FBA喂食锦鲤增加了血清中髓过氧化物酶和FBA特异性抗体的产生。此外，用重组SR21-FBA喂食的锦鲤血清对病原体[病原体名称]表现出显著的杀菌活性。因此，SR21是一种天然且极具前景的口服疫苗载体，它不仅作为功能性饲料成分提供必需营养，还为水生动物提供特异性免疫保护。这种双重应用对于促进水产养殖业的可持续发展至关重要。
