Fernandez-Zafra Teresa, Codeluppi Simone, Uhlén Per
Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden; Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden.
Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.
Exp Cell Res. 2017 Aug 15;357(2):236-242. doi: 10.1016/j.yexcr.2017.06.002. Epub 2017 Jun 3.
Traumatic spinal cord injury is characterized by an initial cell loss that is followed by a concerted cellular response in an attempt to restore the damaged tissue. Nevertheless, little is known about the signaling mechanisms governing the cellular response to injury. Here, we have established an adult ex vivo system that exhibits multiple hallmarks of spinal cord injury and allows the study of complex processes that are difficult to address using animal models. We have characterized the ependymal cell response to injury in this model system and found that ependymal cells can become activated, proliferate, migrate out of the central canal lining and differentiate in a manner resembling the in vivo situation. Moreover, we show that these cells respond to external adenosine triphosphate and exhibit spontaneous Ca activity, processes that may play a significant role in the regulation of their response to spinal cord injury. This model provides an attractive tool to deepen our understanding of the ependymal cell response after spinal cord injury, which may contribute to the development of new treatment options for spinal cord injury.
创伤性脊髓损伤的特征是最初的细胞丢失，随后是一系列细胞反应，试图修复受损组织。然而，关于控制细胞对损伤反应的信号机制知之甚少。在这里，我们建立了一个成年离体系统，该系统表现出脊髓损伤的多个特征，并允许研究使用动物模型难以解决的复杂过程。我们在这个模型系统中表征了室管膜细胞对损伤的反应，发现室管膜细胞可以被激活、增殖、从中央管内衬迁移出来并以类似于体内情况的方式分化。此外，我们表明这些细胞对外源三磷酸腺苷有反应并表现出自发性钙活性，这些过程可能在调节它们对脊髓损伤的反应中起重要作用。该模型为加深我们对脊髓损伤后室管膜细胞反应的理解提供了一个有吸引力的工具，这可能有助于开发脊髓损伤的新治疗方案。
