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通过标记辅助选择培育出的两行制麦芽大麦品种“新佐千穗黄金”，其脂氧合酶-1缺失，可提高啤酒的风味稳定性。

The two-row malting barley cultivar 'New Sachiho Golden' with null lipoxygenase-1 improves flavor stability in beer and was developed by marker-assisted selection.

作者信息

Oozeki Mika, Sotome Toshinori, Haruyama Naoto, Yamaguchi Masahiro, Watanabe Hirohisa, Okiyama Takeshi, Kato Tsuneo, Takayama Toshiyuki, Oyama Makoto, Nagamine Takashi, Suzuki Yasuo, Toyoshima Takako, Sekiwa Takahiro, Oono Kaori, Saito Tetsuya, Usui Masao, Arai Shin, Kumekawa Terunobu, Suzuki Emiko, Shirama Kaori, Kihara Makoto, Hoki Takehiro, Matsubara Hideki, Ohsawa Ryo

机构信息

Tochigi Prefectural Agricultural Experiment Station, 1080 Kawaraya, Utsunomiya, Tochigi 320-0002, Japan.

Faculty of Life and Environmental Science, University of Tsukuba, 1-1-1 Tennohdai, Tsukuba, Ibaraki 305-8572, Japan.

出版信息

Breed Sci. 2017 Mar;67(2):165-171. doi: 10.1270/jsbbs.16104. Epub 2017 Mar 17.

DOI:10.1270/jsbbs.16104
PMID:28588394
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5445967/
Abstract

Lipoxygenase-1 (LOX-1) null 'New Sachiho Golden' is a two-row malting barley ( L.) cultivar released in 2015 that was developed at the Tochigi Prefectural Agricultural Experimental Station by backcross breeding using the high-yield leading cultivar 'Sachiho Golden' as a recurrent parent and the LOX-1 null mutant 'Daikei LM1' as a non-recurrent parent. To develop 'New Sachiho Golden' we used a simple LOX activity assay and marker-assisted selection. This is the first LOX-1 null malting barley cultivar in Japan that is resistant to barley yellow mosaic virus (types I-III). Agronomic characteristics and malting qualities of 'New Sachiho Golden' were similar to those of 'Sachiho Golden', except that 'New Sachiho Golden' had no LOX activity in ungerminated grains and had clearly lower LOX activity during malting than 'Sachiho Golden'. The concentrations of a -2-nonenal (T2N) precursor in wort and beer made from 'New Sachiho Golden' were significantly lower than in those made from 'Sachiho Golden', both before and after storage.

摘要

脂氧合酶-1（LOX-1）缺失的“新佐千穗黄金”是2015年发布的二棱麦芽用大麦（L.）品种，由枥木县农业试验站通过回交育种培育而成，以高产主导品种“佐千穗黄金”为轮回亲本，脂氧合酶-1缺失突变体“大京LM1”为非轮回亲本。为培育“新佐千穗黄金”，我们采用了简单的脂氧合酶活性测定和标记辅助选择。这是日本首个对大麦黄花叶病毒（I-III型）具有抗性的脂氧合酶-1缺失麦芽用大麦品种。“新佐千穗黄金”的农艺性状和麦芽品质与“佐千穗黄金”相似，不同之处在于“新佐千穗黄金”未发芽籽粒中没有脂氧合酶活性，且在制麦过程中的脂氧合酶活性明显低于“佐千穗黄金”。用“新佐千穗黄金”制成的麦芽汁和啤酒中，α-2-壬烯醛（T2N）前体的浓度在储存前后均显著低于用“佐千穗黄金”制成的麦芽汁和啤酒。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19df/5445967/95416b28052f/67_16104_5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19df/5445967/2f457e68a96c/67_16104_1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19df/5445967/92d680a050b5/67_16104_2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19df/5445967/f0099483a014/67_16104_3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19df/5445967/32c955aaa641/67_16104_4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19df/5445967/95416b28052f/67_16104_5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19df/5445967/2f457e68a96c/67_16104_1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19df/5445967/92d680a050b5/67_16104_2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19df/5445967/f0099483a014/67_16104_3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19df/5445967/32c955aaa641/67_16104_4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19df/5445967/95416b28052f/67_16104_5.jpg

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本文引用的文献

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2
Characterization of factors that transform linoleic acid into di- and trihydroxyoctadecenoic acids in mash.对在醪液中将亚油酸转化为二羟基十八碳烯酸和三羟基十八碳烯酸的因素的表征。
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Characterization of lipoxygenase-1 null mutants in barley.大麦中脂氧合酶-1缺失突变体的特性分析
两个现代优质品种“菅井黄金”和“幸穗黄金”中日本麦芽大麦育种的基因组痕迹。
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4
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Breed Sci. 2021 Apr;71(2):277-282. doi: 10.1270/jsbbs.20113. Epub 2021 Feb 25.
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