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缺乏脂氧合酶-1的加拿大麦芽大麦品种“CDC Goldstar”的育种及酿造品质

Breeding and brewing quality of the Canadian malting barley variety 'CDC Goldstar' lacking lipoxygenase-1.

作者信息

Nanamori Masahito, Tokizono Yoshiro, Hoki Takehiro, Saito Wataru, Aritomo Ryota, Yamaki Tsutomu, Hirota Naohiko, Suda Narushi, Beattie Aaron

机构信息

Crop Research Laboratories, Sapporo Breweries Ltd, 37-1 Nittakizaki, Ota, Gunma 370-0321, Japan.

Product & Technology Innovation Department, Sapporo Breweries Ltd, 10 Okatome, Yaizu, Shizuoka 425-0013, Japan.

出版信息

Breed Sci. 2021 Apr;71(2):277-282. doi: 10.1270/jsbbs.20113. Epub 2021 Feb 25.

DOI:10.1270/jsbbs.20113
PMID:34377076
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8329874/
Abstract

Various types of malt quality profiles have been investigated to benefit the North American brewing industry. Herein, we report the development and brewing quality of the hulled, two-row malting barley ( L.) variety 'CDC Goldstar' lacking lipoxygenase-1 (LOX-1-less). This new variety offers a novel malt type for the improvement of beer flavor stability. The agronomic performance of 'CDC Goldstar' was tested in the Western Cooperative Two Row Barley Registration Trials during 2013-2014. In addition to high lodging tolerance, the new variety showed 6% higher yield than the current leading variety 'CDC Copeland'. The malt quality of 'CDC Goldstar' showed higher diastatic power and lower wort β-glucan content than 'CDC Copeland' and controllable proteolytic modification (soluble nitrogen and Kolbach Index). Pilot- (100 L) and commercial-scale (5,000 L) brewing trials were conducted using 'CDC PlatinumStar', another LOX-1-less variety with a low enzymatic profile, as the control variety. Absence of the LOX-1 trait from 'CDC Goldstar' maintained -2-nonenal levels in aged beers as low as those in other LOX-1-less varieties without affecting major beer parameters, such as ester and aldehyde content or foam stability. The newly developed 'CDC Goldstar' malting barley provides added value for the beer industry and consumers.

摘要

为了使北美啤酒酿造业受益,人们对各种类型的麦芽品质特征进行了研究。在此,我们报告了缺乏脂氧合酶-1(无LOX-1)的带壳二棱制麦芽大麦(L.)品种“CDC Goldstar”的培育情况及其酿造品质。这个新品种为改善啤酒风味稳定性提供了一种新型麦芽。2013 - 2014年期间,在西部合作二棱大麦登记试验中对“CDC Goldstar”的农艺性能进行了测试。除了具有高抗倒伏性外,该新品种的产量比当前的主导品种“CDC Copeland”高出6%。“CDC Goldstar”的麦芽品质表现出比“CDC Copeland”更高的糖化力和更低的麦芽汁β-葡聚糖含量,以及可控的蛋白水解修饰(可溶性氮和库尔巴哈值)。以另一个酶谱较低的无LOX-1品种“CDC PlatinumStar”作为对照品种,进行了中试规模(100升)和商业规模(5000升)的酿造试验。“CDC Goldstar”缺乏LOX-1特性,可使陈酿啤酒中的反-2-壬烯醛水平保持在与其他无LOX-1品种一样低的水平,同时不影响主要的啤酒参数,如酯和醛的含量或泡沫稳定性。新培育的“CDC Goldstar”制麦芽大麦为啤酒行业和消费者提供了附加值。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d85/8329874/2dae4138feaf/71_277-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d85/8329874/205e51038dc6/71_277-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d85/8329874/2dae4138feaf/71_277-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d85/8329874/205e51038dc6/71_277-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d85/8329874/2dae4138feaf/71_277-g002.jpg

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本文引用的文献

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Breed Sci. 2017 Mar;67(2):165-171. doi: 10.1270/jsbbs.16104. Epub 2017 Mar 17.
2
Characterization of factors that transform linoleic acid into di- and trihydroxyoctadecenoic acids in mash.对在醪液中将亚油酸转化为二羟基十八碳烯酸和三羟基十八碳烯酸的因素的表征。
J Biosci Bioeng. 2002;93(1):73-7.
3
Characterization of lipoxygenase-1 null mutants in barley.
大麦中脂氧合酶-1缺失突变体的特性分析
Theor Appl Genet. 2005 Nov;111(8):1580-4. doi: 10.1007/s00122-005-0088-y. Epub 2005 Nov 10.
4
Characterization of factors involved in the production of 2(E)-nonenal during mashing.糖化过程中2(E)-壬烯醛生成相关因素的表征
Biosci Biotechnol Biochem. 2003 Apr;67(4):691-7. doi: 10.1271/bbb.67.691.