从韩国一份粪便样本中分离出的CBA7123的全基因组序列。

Complete genome sequence of CBA7123 isolated from a faecal sample from Korea.

作者信息

Kim Yeon Bee, Kim Joon Yong, Song Hye Seon, Lee Changsu, Kwon Joseph, Kang Jisu, Rhee Jin-Kyu, Jeong Myeong Seon, Nam Young-Do, Roh Seong Woon

机构信息

Microbiology and Functionality Research Group, World Institute of Kimchi, Gwangju, 61755 Republic of Korea.

Biological Disaster Analysis Group, Korea Basic Science Institute, Daejeon, 34133 Republic of Korea.

出版信息

Gut Pathog. 2017 Jun 2;9:32. doi: 10.1186/s13099-017-0181-1. eCollection 2017.

DOI:10.1186/s13099-017-0181-1

PMID:28588654

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5457660/

Abstract

BACKGROUND

is an opportunistic human pathogen that causes necrotic enteritis, mild diarrhea, clostridial myonecrosis or gas gangrene, sepsis, etc. In this study, we aim to determine the pathogenesis of this bacterium at the genomic level. The genome of strain CBA7123 was sequenced, and a comparative genomic analysis between strain CBA7123 and four other related strains was performed.

RESULTS

The genome of strain CBA7123 consisted of one circular chromosome and one plasmid that were 3,088,370 and 46,640 bp long with 28.5 and 27.1 mol% G+C content, respectively. The genomic DNA was predicted to contain 2798 open reading frames (ORFs), 10 rRNA genes, and 94 tRNA genes. The genomic comparison analysis between the five strains revealed the distinctive virulence properties of strain CBA7123 by highlighting certain strain-specific genes.

CONCLUSIONS

In this study, the CBA7123 genome was sequenced and compared with other genomes. Among the various genes sequenced, the detection of antimicrobial resistance genes and those encoding various virulence factors may extend the understanding of the pathogenesis of strains.

摘要

背景

是一种机会性人类病原体，可引起坏死性肠炎、轻度腹泻、梭菌性肌坏死或气性坏疽、败血症等。在本研究中，我们旨在从基因组水平确定该细菌的发病机制。对菌株CBA7123的基因组进行了测序，并对菌株CBA7123与其他四个相关菌株进行了比较基因组分析。

结果

菌株CBA7123的基因组由一条环状染色体和一个质粒组成，长度分别为3,088,370和46,640 bp，G+C含量分别为28.5和27.1 mol%。基因组DNA预计包含2798个开放阅读框（ORF）、10个rRNA基因和94个tRNA基因。五个菌株之间的基因组比较分析通过突出某些菌株特异性基因揭示了菌株CBA7123独特的毒力特性。