College of Animal Science and Technology, Jiangxi Agriculture University, Jiangxi, 330045, Nanchang, China.
Sci Rep. 2021 Jan 12;11(1):475. doi: 10.1038/s41598-020-79333-8.
Clostridium perfringens (Cp) is a ubiquitous opportunistic pathogen of humans and animals in the natural environment and animal intestines. The pathogenicity of Cp depends on the production of toxins encoded by genes on the chromosomes or plasmids. In contemporary literature, there is no clear consensus about the pathogenicity of CpA β2 toxin. To analyze the homology of the genome of piglet source CpA and its β2 toxin, we sequenced the whole genome of strain JXJA17 isolated from diarrhea piglets using the Illumina Miseq and Pacbio Sequel platforms. The genome was composed of a circular chromosome with 3,324,072 bp (G + C content: 28.51%) and nine plasmids. Genome and 16S rDNA homology analysis revealed a close relation of the JXJA17 strain with the JGS1495, Cp-06, Cp-16, and FORC_003 strains. These strains were isolated from different samples and belonged to different toxin-types. JXJA17 strain was found to carry two toxin genes (plc and cpb2). In contrast to other Cp strains, the cpb2 of JXJA17 was located on a large plasmid (58 kb) with no co-localization of other toxin genes or antibiotic resistance genes. Analysis of JXJA17 genome homology and its cpb2 would facilitate our further study the relationship between β2 toxin and piglet diarrhea.
产气荚膜梭菌（Cp）是一种普遍存在的机会致病菌，存在于自然环境和动物肠道中的人类和动物中。Cp 的致病性取决于染色体或质粒上基因编码的毒素的产生。在当代文献中，对于 CpAβ2 毒素的致病性尚无明确共识。为了分析仔猪源 CpA 的基因组与其β2 毒素的同源性，我们使用 Illumina Miseq 和 Pacbio Sequel 平台对从腹泻仔猪中分离出的菌株 JXJA17 进行了全基因组测序。基因组由一个 3,324,072bp 的圆形染色体（G+C 含量：28.51%）和九个质粒组成。基因组和 16S rDNA 同源性分析表明，JXJA17 菌株与 JGS1495、Cp-06、Cp-16 和 FORC_003 菌株密切相关。这些菌株是从不同的样本中分离出来的，属于不同的毒素型。JXJA17 菌株被发现携带两个毒素基因（plc 和 cpb2）。与其他 Cp 菌株不同，JXJA17 的 cpb2 位于一个没有其他毒素基因或抗生素抗性基因共存的大质粒（58kb）上。分析 JXJA17 基因组的同源性及其 cpb2 有助于我们进一步研究β2 毒素与仔猪腹泻之间的关系。
