Granum P E
Acta Pathol Microbiol Immunol Scand B. 1985 Feb;93(1):41-8. doi: 10.1111/j.1699-0463.1985.tb02849.x.
Clostridium perfringens enterotoxin binds to receptors on Vero cells. This process does not depend on the presence of divalent cations (Ca++, Mg++). Binding of enterotoxin causes inhibition of 14C-leucine incorporation into proteins, probably because of depression of amino acid transport. The presence of Mg++ speeds up this effect of the enterotoxin. The enterotoxin produces membrane leakage only in the presence of Ca++, but additional Mg++ increases the rate of this process. These results indicate that the dissociation constant of the enterotoxin receptor interaction is reduced in the presence of Mg++. A model for the mode of action of the enterotoxin is proposed.
产气荚膜梭菌肠毒素与非洲绿猴肾细胞（Vero细胞）上的受体结合。这一过程不依赖于二价阳离子（Ca++、Mg++）的存在。肠毒素的结合会抑制14C-亮氨酸掺入蛋白质，这可能是由于氨基酸转运受抑制所致。Mg++的存在会加速肠毒素的这种作用。肠毒素仅在Ca++存在的情况下才会导致细胞膜渗漏，但额外的Mg++会加快这一过程的速率。这些结果表明，在Mg++存在的情况下，肠毒素受体相互作用的解离常数会降低。本文提出了肠毒素作用模式的模型。
