产气荚膜梭菌的分子遗传学与发病机制
Molecular genetics and pathogenesis of Clostridium perfringens.
作者信息
Rood J I, Cole S T
机构信息
Department of Microbiology, Monash University, Clayton, Victoria, Australia.
出版信息
Microbiol Rev. 1991 Dec;55(4):621-48. doi: 10.1128/mr.55.4.621-648.1991.
Clostridium perfringens is the causative agent of a number of human diseases, such as gas gangrene and food poisoning, and many diseases of animals. Recently significant advances have been made in the development of C. perfringens genetics. Studies on bacteriocin plasmids and conjugative R plasmids have led to the cloning and analysis of many C. perfringens genes and the construction of shuttle plasmids. The relationship of antibiotic resistance genes to similar genes from other bacteria has been elucidated. A detailed physical map of the C. perfringens chromosome has been prepared, and numerous genes have been located on that map. Reproducible transformation methods for the introduction of plasmids into C. perfringens have been developed, and several genes coding for the production of extracellular toxins and enzymes have been cloned. Now that it is possible to freely move genetic information back and forth between C. perfringens and Escherichia coli, it will be possible to apply modern molecular methods to studies on the pathogenesis of C. perfringens infections.
产气荚膜梭菌是多种人类疾病的病原体，如气性坏疽和食物中毒，以及许多动物疾病的病原体。最近，产气荚膜梭菌遗传学的发展取得了重大进展。对细菌素质粒和接合性R质粒的研究导致了许多产气荚膜梭菌基因的克隆和分析以及穿梭质粒的构建。已经阐明了抗生素抗性基因与其他细菌相似基因的关系。已经制备了产气荚膜梭菌染色体的详细物理图谱，并且许多基因已定位在该图谱上。已经开发出将质粒导入产气荚膜梭菌的可重复转化方法，并且已经克隆了几个编码细胞外毒素和酶产生的基因。既然现在可以在产气荚膜梭菌和大肠杆菌之间自由地来回转移遗传信息，那么就有可能将现代分子方法应用于产气荚膜梭菌感染发病机制的研究。
Zotero 插件