Field James Clark, Waterhouse Paula Jane, German Matthew John
Newcastle University.
Eur J Prosthodont Restor Dent. 2017 Jun;25(2):93-100. doi: 10.1922/EJPRD_01660Field08.
This study aimed to test the null hypothesis that there are no significant differences in surface characteristics of eroded and abraded human, ovine and bovine enamel.
Twenty enamel slabs were prepared from bovine, human and ovine incisor crowns, and randomly assigned to one of 4 treatments: 30 second/4 minute immersion at 1%/6% w/v citric acid. Post-erosion, an oscillatory brush was used for 20 seconds. Roughness parameters, height change and scanning electron microscopy were measured at each stage.
Whilst the eroded surface became generally less rough after the abrasive challenge, there were significant tissue differences. Abraded microhardness was significantly increased compared to eroded values (P⟨0.001), Surface loss was also significantly different between tissue types (P⟨0.001). Bovine enamel showed similar trends to human enamel but was consistently harder and more resistant to surface change. Ovine enamel displayed little correlation with human enamel.Scanning electron microscopy images showed differences for each tissue that were consistent with the quantitative data.
The null hypothesis was rejected; neither bovine nor ovine enamel can be reliably used interchangeably with human enamel for erosion/abrasion studies.
本研究旨在检验零假设，即侵蚀和磨损后的人类、绵羊和牛的牙釉质表面特征不存在显著差异。
从牛、人类和绵羊的切牙冠制备20个牙釉质块，并随机分配到4种处理之一：在1%/6% w/v柠檬酸中浸泡30秒/4分钟。侵蚀后，使用振荡刷处理20秒。在每个阶段测量粗糙度参数、高度变化和扫描电子显微镜。
虽然在磨损挑战后，侵蚀表面通常变得不那么粗糙，但存在显著的组织差异。与侵蚀值相比，磨损后的显微硬度显著增加（P⟨0.001），组织类型之间的表面损失也存在显著差异（P⟨0.001）。牛牙釉质与人类牙釉质呈现相似趋势，但始终更硬且更耐表面变化。绵羊牙釉质与人类牙釉质几乎没有相关性。扫描电子显微镜图像显示各组织的差异与定量数据一致。
零假设被拒绝；在侵蚀/磨损研究中，牛和绵羊的牙釉质都不能可靠地与人类牙釉质互换使用。
