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人类恒牙、牛牙和猪牙釉质的比较扫描电子显微镜分析。

Comparative scanning electron microscope analysis of the enamel of permanent human, bovine and porcine teeth.

作者信息

Olek Aneta, Klimek Leszek, Bołtacz-Rzepkowska Elżbieta

机构信息

Department of Conservative Dentistry, Medical University of Lodz, 92-217 Łódź, Poland.

Institute of Materials Science and Engineering, University of Technology in Lodz, 90-924 Łódź, Poland.

出版信息

J Vet Sci. 2020 Nov;21(6):e83. doi: 10.4142/jvs.2020.21.e83.

DOI:10.4142/jvs.2020.21.e83
PMID:33263230
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7710455/
Abstract

BACKGROUND

Bovine and porcine teeth are often used in experiments as substitutes of human teeth.

OBJECTIVES

The aim of the present study was to perform a comparative analysis of enamel morphology of permanent human, bovine and porcine teeth under the scanning electron microscope.

METHODS

As many as 10 human, 10 bovine, and 10 porcine teeth were studied. All the teeth were sectioned and the halves were randomly divided into 2 groups according to the examined tissue (vestibular enamel at the mid-height of the dental crown and in the cervical area). Human and bovine enamel was etched for 15 sec and porcine enamel for 30 sec. The scanning electron microscope analysis was performed. The length and width of enamel prisms were determined with the "Met-Ilo" 1.1 computer program.

RESULTS

All enamel samples revealed the same etching pattern-Silverstone's type 2. Bovine enamel showed a similar porosity and the amount of interprismatic enamel compared to human enamel while the amount and width of interprismatic enamel bands in porcine enamel were evidently greater. The shape of the porcine prisms was visually similar to human prisms, although dimensions were significantly different. However, bovine prisms differed in form and appeared to be distinctly elongated.

CONCLUSIONS

Reported findings indicate that the results of experimental studies carried out on bovine and porcine enamel should not be compared with the results obtained on human enamel.

摘要

背景

牛牙和猪牙常用于实验中作为人牙的替代品。

目的

本研究旨在在扫描电子显微镜下对恒牙期的人牙、牛牙和猪牙的釉质形态进行对比分析。

方法

研究了10颗人牙、10颗牛牙和10颗猪牙。将所有牙齿进行切片,根据检查组织(牙冠中部高度和颈部区域的前庭釉质)将半颗牙齿随机分为2组。人牙和牛牙的釉质蚀刻15秒,猪牙的釉质蚀刻30秒。进行扫描电子显微镜分析。用“Met-Ilo”1.1计算机程序测定釉柱的长度和宽度。

结果

所有釉质样本均呈现相同的蚀刻模式——西尔弗斯通2型。与人类釉质相比,牛釉质显示出相似的孔隙率和柱间质釉质的量,而猪釉质中柱间质釉质带的量和宽度明显更大。猪釉柱的形状在视觉上与人釉柱相似,尽管尺寸有显著差异。然而,牛釉柱在形态上不同,似乎明显拉长。

结论

报告的研究结果表明,对牛釉质和猪釉质进行的实验研究结果不应与人釉质的实验结果进行比较。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0526/7710455/0cdad96d8d4f/jvs-21-e83-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0526/7710455/d0f24878a775/jvs-21-e83-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0526/7710455/15618fe9b7db/jvs-21-e83-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0526/7710455/ec878f57baee/jvs-21-e83-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0526/7710455/49f140cddafc/jvs-21-e83-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0526/7710455/056e386dd36c/jvs-21-e83-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0526/7710455/0cdad96d8d4f/jvs-21-e83-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0526/7710455/d0f24878a775/jvs-21-e83-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0526/7710455/15618fe9b7db/jvs-21-e83-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0526/7710455/ec878f57baee/jvs-21-e83-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0526/7710455/49f140cddafc/jvs-21-e83-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0526/7710455/056e386dd36c/jvs-21-e83-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0526/7710455/0cdad96d8d4f/jvs-21-e83-g006.jpg

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