亚洲玉米螟对Bt毒素Cry1Ie抗性的鉴定
Characterization of Asian Corn Borer Resistance to Bt Toxin Cry1Ie.
作者信息
Wang Yueqin, Yang Jing, Quan Yudong, Wang Zhenying, Cai Wanzhi, He Kanglai
机构信息
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Department of Entomology, China Agricultural University, Beijing 100193, China.
出版信息
Toxins (Basel). 2017 Jun 7;9(6):186. doi: 10.3390/toxins9060186.
A strain of the Asian corn borer (ACB), (Guenée), has evolved >800-fold resistance to Cry1Ie (ACB-IeR) after 49 generations of selection. The inheritance pattern of resistance to Cry1Ie in ACB-IeR strain and its cross-resistance to other Bt toxins were determined through bioassay by exposing neonates from genetic-crosses to toxins incorporated into the diet. The response of progenies from reciprocal F₁ crosses were similar (LCs: 76.07 vs. 74.32 μg/g), which suggested the resistance was autosomal. The effective dominance () decreased as concentration of Cry1Ie increased. was nearly recessive or incompletely recessive on Cry1Ie maize leaf tissue ( = 0.02), but nearly dominant or incompletely dominant ( = 0.98) on Cry1Ie maize silk. Bioassay of the backcross suggested that the resistance was controlled by more than one locus. In addition, the resistant strain did not perform cross-resistance to Cry1Ab (0.8-fold), Cry1Ac (0.8-fold), Cry1F (0.9-fold), and Cry1Ah (1.0-fold). The present study not only offers the manifestation for resistance management, but also recommends that Cry1Ie will be an appropriate candidate for expression with Cry1Ab, Cry1Ac, Cry1F, or Cry1Ah for the development of Bt maize.
亚洲玉米螟（Asian corn borer，ACB）的一个品系，即谷螟（Guenée），在经过49代选择后，对Cry1Ie产生了超过800倍的抗性（ACB-IeR）。通过将遗传杂交产生的初孵幼虫暴露于掺入饲料中的毒素进行生物测定，确定了ACB-IeR品系对Cry1Ie的抗性遗传模式及其对其他Bt毒素的交叉抗性。正反交F₁代杂交后代的反应相似（LC₅₀：76.07对74.32 μg/g），这表明抗性是常染色体遗传。随着Cry1Ie浓度的增加，有效显性度（D）降低。在Cry1Ie玉米叶片组织上，D几乎是隐性或不完全隐性的（D = 0.02），但在Cry1Ie玉米花丝上几乎是显性或不完全显性的（D = 0.98）。回交生物测定表明，抗性由多个基因座控制。此外，抗性品系对Cry1Ab（0.8倍）、Cry1Ac（0.8倍）、Cry1F（0.9倍）和Cry1Ah（1.0倍）没有交叉抗性。本研究不仅为抗性管理提供了表现形式，还建议Cry1Ie将是与Cry1Ab、Cry1Ac、Cry1F或Cry1Ah一起表达用于开发Bt玉米的合适候选者。
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