Henan Key Laboratory of Crop Pest Control, Key Laboratory of Integrated Pest Management on Crops in the Southern Region of North China, Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou, China.
Department of Entomology, College of Plant Protection, Henan Agricultural University, Zhengzhou, China.
Pest Manag Sci. 2020 Dec;76(12):4311-4317. doi: 10.1002/ps.5999. Epub 2020 Jul 31.
Ostrinia furnacalis (Guenée) is one of the most destructive pests of corn and is a major target of transgenic corn expressing Bt (Bacillus thuringiensis) toxins in the Huanghuaihai summer corn region of China. Prior to the widespread commercialization of transgenic Bt corn, it is necessary to estimate baseline susceptibility to Bt toxins and Bt toxin resistance allele frequencies in O. furnacalis.
The median lethal concentration (LC ) values of the Bt toxins Cry1Ab, Cry1Ac and Cry1F for 15 different populations ranged from 0.887 to 1.617, 1.251 to 2.594 and 4.146 to 6.465 ng cm , respectively. The LC values of 93, 45, and 197 ng cm for Cry1Ab, Cry1Ac and Cry1F, respectively, killed > 99% of individuals of eight O. furnacalis populations collected in 2017 and were identified as diagnostic concentrations for monitoring susceptibility in O. furnacalis populations in this region. Using the F screening method with these diagnostic concentrations, the resistance allele frequencies related to Cry1Ab, Cry1Ac and Cry1F were found to be 0.002 (0.000283-0.006484), 0.001 (0.000030-0.004295) and 0.001 (0.000030-0.004295), respectively, in 2018.
Fifteen populations of O. furnacalis collected in the Huanghuaihai summer corn region of China were all susceptible to Cry1Ab, Cry1Ac and Cry1F toxins, and the susceptibility showed no significant variation among these O. furnacalis populations. The estimated resistance allele frequency to Cry1Ab, Cry1Ac and Cry1F was rare in this region. This provided essential knowledge for making the decision to commercialize Bt maize, and monitoring resistance development and evaluating resistance management strategies in the future in the Huanghuaihai summer corn region of China. © 2020 Society of Chemical Industry.
亚洲玉米螟（Ostrinia furnacalis (Guenée)）是玉米的最具破坏性害虫之一，也是中国黄淮海夏玉米区表达苏云金芽孢杆菌（Bacillus thuringiensis）毒素的转基因玉米的主要靶标。在转基因抗虫玉米广泛商业化之前，有必要估计亚洲玉米螟对苏云金毒素的基础敏感性和 Bt 毒素抗性等位基因频率。
15 个不同种群的 Bt 毒素 Cry1Ab、Cry1Ac 和 Cry1F 的中致死浓度（LC ）值范围分别为 0.887-1.617、1.251-2.594 和 4.146-6.465 ng cm 。Cry1Ab、Cry1Ac 和 Cry1F 的 LC 值分别为 93、45 和 197 ng cm ，可杀死 2017 年采集的 8 个亚洲玉米螟种群中 >99%的个体，被鉴定为该地区监测亚洲玉米螟种群敏感性的诊断浓度。使用这些诊断浓度的 F 筛选法，发现与 Cry1Ab、Cry1Ac 和 Cry1F 相关的抗性等位基因频率分别为 0.002（0.000283-0.006484）、0.001（0.000030-0.004295）和 0.001（0.000030-0.004295）。2018 年。
在中国黄淮海夏玉米区采集的 15 个亚洲玉米螟种群均对 Cry1Ab、Cry1Ac 和 Cry1F 毒素敏感，这些亚洲玉米螟种群之间的敏感性无显著差异。该地区对 Cry1Ab、Cry1Ac 和 Cry1F 的估计抗性等位基因频率很少。这为在中国黄淮海夏玉米区商业化种植 Bt 玉米、监测抗药性发展以及评估未来抗药性管理策略提供了必要的知识。© 2020 化学工业协会。
