Tomazella Iara Maluf, Abril Vanessa Veltrini, Duarte José Maurício Barbanti
Universidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Câmpus Jaboticabal, Departamento de Zootecnia, Núcleo de Pesquisa e Conservação de Cervídeos (NUPECCE), Jaboticabal, SP, Brazil.
Universidade Federal de Mato Grosso (UFMT), Campus Universitário do Araguaia, Instituto de Ciências Biológicas e da Saúde (ICBS), Pontal do Araguaia, MT, Brazil.
Genet Mol Biol. 2017 Apr-Jun;40(2):460-467. doi: 10.1590/1678-4685-GMB-2016-0275. Epub 2017 Jun 5.
The process of karyotype evolution in Cervidae from a common ancestor (2n = 70, FN = 70) has been marked by complex chromosomal rearrangements. This ancestral karyotype has been retained by the current species Mazama gouazoubira (Fischer 1814), for which a chromosomal polymorphism (Robertsonian translocations and the presence of B chromosomes) has been described, presumably caused by a chromosome fragility. Thus, this study has identified doxorubicin-induced chromosome aberrations and mapped the regions involved in breaks, which may be related to the chromosome evolution process. G-banding pattern showed that 21 pairs of chromosomes presented chromosomal aberrations, 60% of the total chromosome number of the species M. gouazoubira. Among chromosomes that carry aberrations, the region where they were most frequently concentrated was distal relative to the centromere. These data suggest that certain chromosomal regions may be more susceptible to chromosome fragility and consequently could be involved in karyotype differentiation in species of the family Cervidae.
鹿科从共同祖先（2n = 70，FN = 70）开始的核型进化过程以复杂的染色体重排为特征。当前物种马泽马鹿（Mazama gouazoubira，费舍尔，1814年）保留了这种祖先核型，该物种已被描述存在染色体多态性（罗伯逊易位和B染色体的存在），推测是由染色体脆弱性引起的。因此，本研究鉴定了阿霉素诱导的染色体畸变，并绘制了涉及断裂的区域，这些区域可能与染色体进化过程有关。G带模式显示，21对染色体出现了染色体畸变，占马泽马鹿物种染色体总数的60%。在携带畸变的染色体中，它们最常集中的区域相对于着丝粒位于远端。这些数据表明，某些染色体区域可能更容易受到染色体脆弱性的影响，因此可能参与鹿科物种的核型分化。
