Gülkaç Mehmet Dogan, Akpinar Gürler, Ustün Hasan, Ozön Kanli Aylin
Department of Medical Biology, Faculty of Medicine, Kocaeli University, 41900 Derince-Kocaeli, Turkey.
Mutagenesis. 2004 May;19(3):231-6. doi: 10.1093/mutage/geh021.
The present study was carried out to evaluate the role of vitamin A (VA) on the induction of chromosomal aberrations (CA) in rat bone marrow cells and to investigate its modulating effect on chromosomal damage induced by doxorubicin (DXR). Wistar rats were treated with VA (7.5, 15 and 30 microg/kg body wt) once a day for 2 days by gavage before injecting DXR (90 mg/kg body wt). Rats in the control group were treated with corresponding doses of water and olive oil. Animals treated with the medium dose of VA (15 microg/kg body wt) plus single dose of DXR presented a statistically significant reduction in total number of CA and in number of abnormal metaphases (P < 0.05). However, when compared with control and DXR groups, the low and high VA doses (7.5 and 30 microg/kg body wt) were found to be less efficient than the medium dose VA (15 microg/kg body wt) in terms of parameters analyzed. Furthermore, the high dose of VA group (30 microg/kg body wt) was found to be clastogenic (P < 0.05). This study concludes that the protective effect of VA against chromosome damage is dose dependent.
本研究旨在评估维生素A（VA）对大鼠骨髓细胞染色体畸变（CA）诱导的作用，并研究其对阿霉素（DXR）诱导的染色体损伤的调节作用。在注射DXR（90 mg/kg体重）前，Wistar大鼠每天经口灌胃给予VA（7.5、15和30 μg/kg体重），持续2天。对照组大鼠给予相应剂量的水和橄榄油。给予中等剂量VA（15 μg/kg体重）加单次剂量DXR治疗的动物，其CA总数和异常中期相数量在统计学上显著减少（P < 0.05）。然而，就所分析的参数而言，与对照组和DXR组相比，低剂量和高剂量VA（7.5和30 μg/kg体重）的效果不如中等剂量VA（15 μg/kg体重）。此外，高剂量VA组（30 μg/kg体重）具有致断裂作用（P < 0.05）。本研究得出结论，VA对染色体损伤的保护作用具有剂量依赖性。
