Observations on cell growth and regulation of glutamine synthetase by dexamethasone in primary cultures of forebrain and cerebellar astrocytes.

Cell growth, development of glutamine synthetase and its regulation by glucocorticoids, were studied in primary cultures of two types of astrocytes derived from rat brain, one from newborn forebrain and another from either newborn or 8-day-old cerebellum. Cell number per dish increased linearly following an initial decrease due to removal of non-astrocytic cells, and after the second week reached a stationary phase in both types of cultures at more or less the same time, although the cell number in cerebellar cultures was about 35% lower than in forebrain cultures of the same age. At all ages, irrespective of cell density, the cerebellar astrocytes were larger in size than the forebrain astroglial cells. The developmental curves for glutamine synthetase activity were similar in vitro and in vivo; however, the increase in enzyme activity in vitro was significantly greater than in vivo and this difference was more marked in forebrain than in cerebellar cultures. Throughout the period studied the specific activity of glutamine synthetase was significantly higher in forebrain astrocytes than in cerebellar astroglial cells. Treatment with dexamethasone caused a marked increase in the specific activity of glutamine synthetase. However, in agreement with our previous in vivo findings, the steroid induction in forebrain astrocytes was significantly less than that in cerebellar astrocytes. In culture, both types of astrocytes remain responsive to the hormone for longer than in vivo. The differences in the biochemical properties of the forebrain and cerebellar astrocytes seem to be intrinsic, and not related to the cell density or to the purity of the cultures.