Khan M M, Melmon K L, Fathman C G, Hertel-Wulff B, Strober S
J Immunol. 1985 Jun;134(6):4100-6.
The pharmacologic effects of histamine and isoproterenol (autacoids) were studied on clones of murine T helper (TH) and natural suppressor (NS) cells. The data are consistent with receptors for the autacoids being nonrandomly distributed on phenotypically and functionally distinct clones. The effects of histamine on IL 2 secretion by TH cells could be either inhibitory or stimulatory, depending on the conditions of incubation with the autacoid. When TH cells were pretreated with histamine, their secretion of IL 2 was augmented; conversely, if histamine was added to the TH cells in the presence of antigen, IL 2 secretion was inhibited. The suppressor function of NS cells was enhanced by preincubation with histamine (10(-4) M) for 4 hr before the washed NS cells were added to responder and stimulator cells in mixed lymphocyte cultures. Two H1 receptor antagonists, mepyramine (10(-6) M) and pyrobutamine (10(-7) M), each competitively blocked the histamine activation of the NS cells, but an H2 receptor antagonist cimetidine (10(-5) M) did not alter the suppressor-enhancing function of histamine. Activation of NS cells did not occur if histamine was added with responder, stimulator, and co-cultured cells in MLR. The effects of each autacoid were additive in cytolytic T cells alone. The adenylate cyclase pools that can be stimulated by isoproterenol and histamine in cytolytic T cells may be independent of each other, but further work will be needed to prove this point.
研究了组胺和异丙肾上腺素（自体活性物质）对小鼠T辅助（TH）细胞克隆和天然抑制（NS）细胞克隆的药理作用。数据表明，这些自体活性物质的受体在表型和功能不同的克隆上呈非随机分布。组胺对TH细胞分泌白细胞介素2（IL - 2）的影响可能是抑制性的，也可能是刺激性的，这取决于与自体活性物质孵育的条件。当TH细胞用组胺预处理时，它们的IL - 2分泌增加；相反，如果在有抗原存在的情况下将组胺添加到TH细胞中，IL - 2分泌则受到抑制。在混合淋巴细胞培养中，将洗涤后的NS细胞添加到反应细胞和刺激细胞之前，用组胺（10⁻⁴ M）预孵育4小时可增强NS细胞的抑制功能。两种H1受体拮抗剂，美吡拉敏（10⁻⁶ M）和吡丁胺（10⁻⁷ M），均可竞争性阻断组胺对NS细胞的激活作用，但H2受体拮抗剂西咪替丁（10⁻⁵ M）并未改变组胺的抑制增强功能。如果在混合淋巴细胞反应（MLR）中将组胺与反应细胞、刺激细胞和共培养细胞一起添加，则不会发生NS细胞的激活。在单独的细胞毒性T细胞中，每种自体活性物质的作用是相加的。在细胞毒性T细胞中，可被异丙肾上腺素和组胺刺激的腺苷酸环化酶池可能彼此独立，但需要进一步的研究来证明这一点。
